ArticleMycorrhiza2014
Simultaneous specific in planta visualization of root-colonizing fungi using fluorescence in situ hybridization (FISH).
Article in Mycorrhiza, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Cellular anatomy of arbuscular mycorrhizal fungi.Current biology : CB · 2025Review
- Newly Designed Fluorescence In Situ Hybridization Probes Reveal Previously Unknown Endophytic Abilities of Tuber magnatum in Herbaceous Plants.Microbial ecology · 2025Article
- Exploring the potential of endophytes and their metabolites for bio-control activity.3 Biotech · 2022Review
- Investigating plant-microbe interactions within the root.Archives of microbiology · 2022Review
- Micro-scale Experimental System Coupled with Fluorescence-based Estimation of Fungal Biomass to Study Utilisation of Plant Substrates.Microbial ecology · 2022Article
- Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives.Frontiers in microbiology · 2021Article
- Unravelling the Role of Melanin in Cd and Zn Tolerance and Accumulation of Three Dark Septate Endophytic Species.Microorganisms · 2020Article
- Detecting the colonization of ericoid mycorrhizal fungi inPlant methods · 2020Article
- Considerations and consequences of allowing DNA sequence data as types of fungal taxa.IMA fungus · 2018Article
- Co-inoculation of Lolium perenne with Funneliformis mosseae and the dark septate endophyte Cadophora sp. in a trace element-polluted soil.Mycorrhiza · 2018Article
- The Mycelium as a Network.Microbiology spectrum · 2017Review
- In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections.Plant methods · 2016Article
- Mycobiome detection from a single subterranean gametophyte using metabarcoding techniques.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In planta detection of mutualistic, endophytic, and pathogenic fungi commonly colonizing roots and other plant organs is not a routine task. We aimed to use fluorescence in situ hybridization (FISH) for simultaneous specific detection of different fungi colonizing the same tissue. We have adapted ribosomal RNA (rRNA) FISH for visualization of common mycorrhizal (arbuscular- and ectomycorrhiza) and endophytic fungi within roots of different plant species. Beside general probes, we designed and used specific ones hybridizing to the large subunit of rRNA with fluorescent dyes chosen to avoid or reduce the interference with the autofluorescence of plant tissues. We report here an optimized efficient protocol of rRNA FISH and the use of both epifluorescence and confocal laser scanning microscopy for simultaneous specific differential detection of those fungi colonizing the same root. The method could be applied for the characterization of other plant-fungal interactions, too. In planta FISH with specific probes labeled with appropriate fluorescent dyes could be used not only in basic research but to detect plant colonizing pathogenic fungi in their latent life-period.
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