ArticleParasitology research2014
Comparison between two commercially available serological tests and polymerase chain reaction in the diagnosis of Cryptosporidium in animals and diarrhoeic children.
Article in Parasitology research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Child exposure to animal feces and zoonotic pathogens in northwest Ecuador: A mixed-methods study.PLoS neglected tropical diseases · 2026Article
- Cryptosporidiosis diagnosed using metagenomic next-generation sequencing in a healthy child admitted to pediatric intensive care unit: a case report.Frontiers in cellular and infection microbiology · 2023Article
- Novel Dietary Approach with Probiotics, Prebiotics, and Synbiotics to Mitigate Antimicrobial Resistance and Subsequent Out Marketplace of Antimicrobial Agents: A Review.Infection and drug resistance · 2023Review
- Article
- Cryptosporidiosis: From Prevention to Treatment, a Narrative Review.Microorganisms · 2022Review
- Development and Preliminary Application of a Triplex Real-Time Quantitative PCR Assay for the Simultaneous Detection ofFrontiers in microbiology · 2022Article
- Review
- Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection ofJournal of tropical medicine · 2018Article
- A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt.Pathogens (Basel, Switzerland) · 2017Review
- Cryptosporidium and Giardia in Africa: current and future challenges.Parasites & vectors · 2017Review
- Article
- Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016Article
- Cryptosporidium infection in solid organ transplantation.World journal of transplantation · 2016Review
- Highly specific detection of Cryptosporidium spp. oocysts in human stool samples by undemanding and inexpensive phase contrast microscopy.Parasitology research · 2016Article
- Simultaneous detection of Entamoeba histolytica/dispar, Giardia duodenalis and cryptosporidia by immunochromatographic assay in stool samples from patients living in the Greater Cairo Region, Egypt.World journal of microbiology & biotechnology · 2015Article
- Evaluation of ImmunoCard STAT test and ELISA versus light microscopy in diagnosis of giardiasis and cryptosporidiosis.Parasitology research · 2015Article
- Enteric protozoa of cats and their zoonotic potential-a field study from Austria.Parasitology research · 2015Article
- Frequencies and spatial distributions of Cryptosporidium in livestock animals and children in the Ismailia province of Egypt.Epidemiology and infection · 2015Article
- Development of an immunomagnetic bead separation-coupled quantitative PCR method for rapid and sensitive detection of Cryptosporidium parvum oocysts in calf feces.Parasitology research · 2014Article
- Revisiting the global problem of cryptosporidiosis and recommendations.Tropical parasitologyArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the detection of Cryptosporidium species in 804 animals and 165 diarrhoeic children (<10 years) in Egypt, two copro-antigen tests, the RIDASCREEN® Cryptosporidium test [enzyme immunoassay (EIA)] and the RIDA®QUICK Cryptosporidium/Giardia Combi [immuno-chromatographic test (ICT)] as well as polymerase chain reaction (PCR) were used. Prevalence of Cryptosporidium was 15.0, 19.5 and 32.3% in animals and 2.4, 6.7 and 49.1% in children using EIA, ICT and PCR, respectively.Using PCR as reference method, animal samples sensitivity (Se) of the EIA was 46.5% when questionable samples were considered positive, whereas specificity (Sp) was 100%. Se of the ICT was 60.4% while Sp was 100%. Positive predictive values (PPVs) for both EIA and ICT test were 100%, and negative predictive values (NPVs) for EIA were 79.7 and 84.1% for ICT. For the children samples, the Se of EIA was 5%, Sp was 100%, PPV was 100% and NPV was 52.2%, while the Se of ICT was 13.6%, Sp was 100%, PPV was 100% and NPV was 54.6%.The Kappa score of agreement between PCR and ICT was 67.4%, 54.1% between PCR and EIA and 84.4% between ICT and EIA. Until the second serial dilution of the EIA and ICT test, 9 × 10(3) oocysts/μl of Cryptosporidia was detected, whereas in PCR, they were detected until the sixth serial dilution. Copro-antigen tests were easy to perform and less time-consuming but less sensitive compared to PCR. They obviously are best applicable for screening and epidemiological studies of large numbers of subjects, for batch specimen processing and in isolated or rural areas where reliable tests like PCR are unfeasible. When in children, a single stool sample is used for the diagnosis of clinical cases; better results can be obtained when non-standardized PCR due low specificity is coupled with copro-antigen tests.
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