ArticleThe Journal of cell biology1986
Immunolocalization of the gamma isoform of nonmuscle actin in cultured cells.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 114 citations in OpenAlex.
- Mechanics of blunting of actin-myosin interaction dynamics by the actinopathy-causing mutation E334Q in cytoskeletal γ-actin.The Journal of physiology · 2026Article
- Nucleotide- and Protein-Dependent Functions ofMolecular biology of the cell · 2022Article
- Regulation of actin isoforms in cellular and developmental processes.Seminars in cell & developmental biology · 2020Review
- Neutrophil-like HL-60 cells expressing only GFP-tagged β-actin exhibit nearly normal motility.Cytoskeleton (Hoboken, N.J.) · 2020Article
- Expression of trans-membrane serine protease 3 (TMPRSS3) in the human organ of Corti.Cell and tissue research · 2018Article
- The makings of the 'actin code': regulation of actin's biological function at the amino acid and nucleotide level.Journal of cell science · 2018Review
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
- Article
- Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells.Histochemistry and cell biology · 2014Article
- Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens.Proteome science · 2012Article
- β-Actin specifically controls cell growth, migration, and the G-actin pool.Molecular biology of the cell · 2011Article
- Article
- The actin gene family: function follows isoform.Cytoskeleton (Hoboken, N.J.) · 2010Review
- Differential arginylation of actin isoforms is regulated by coding sequence-dependent degradation.Science (New York, N.Y.) · 2010Article
- Delayed embryonic development and impaired cell growth and survival in Actg1 null mice.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Gamma-actin is required for cytoskeletal maintenance but not development.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Arginyltransferase regulates alpha cardiac actin function, myofibril formation and contractility during heart development.Development (Cambridge, England) · 2008Article
- Microtubule asymmetry during neutrophil polarization and migration.Molecular biology of the cell · 2002Article
- Neurotrophin regulation of beta-actin mRNA and protein localization within growth cones.The Journal of cell biology · 1999Article
- Sorting of beta-actin mRNA and protein to neurites and growth cones in culture.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
In many vertebrate nonmuscle cells, the microfilament subunit protein, actin, exists as two isoforms, called beta and gamma, whose sequences differ only in their amino-terminal regions. We have prepared a peptide antibody specifically reactive with the amino-terminal sequence of gamma actin. This antibody reacted with nonmuscle actin as determined by Western blots of SDS gels, and reacted with the gamma, but not the beta, nonmuscle actin isoform as shown by Western blots of isoelectric focusing gels. In immunofluorescence experiments, the gamma peptide antibody stained microfilament bundles, ruffled edges, and the contractile ring of a variety of cultured cells, including mouse L cells, which have previously been reported to contain only the beta actin isoform (Sakiyama, S., S. Fujimura, and H. Sakiyama, 1981, J. Biol. Chem., 256:31-33). Double immunofluorescence experiments using the gamma peptide antibody and an antibody reactive with all actin isoforms revealed no differences in isoform localization. Thus, at the level of resolution of light microscopy, we have detected the gamma actin isoform in all microfilament-containing structures in cultured cells, and have observed no subcellular sorting of the nonmuscle actin isoforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.