ArticlePlant cell reports1991
Relationship between protoplast size and critical field strength in protoplast electropulsing and application to reliable DNA uptake in Brassica.
Article in Plant cell reports, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed.
- PEGylated Positively Charged Fluorescent Silica Nanoparticles for Enhanced Gene Delivery in Canola (ACS omega · 2026Article
- Optimization of a rapid, sensitive, and high throughput molecular sensor to measure canola protoplast respiratory metabolism as a means of screening nanomaterial cytotoxicity.Plant methods · 2024Article
- The effect of temperature conditions during growth on the transformation frequency ofBiochemistry and biophysics reports · 2022Article
- Electroporation modulates the embryogenic responses of asparagus (Asparagus officinalis L.) microspores.Protoplasma · 2001Article
- Energy dissipation as a key factor for electroporation of protoplasts.Molecular biotechnology · 1998Article
- Rapid optimization of electroporation conditions for plant cells, protoplasts, and pollen.Molecular biotechnology · 1995Article
- Transient gene expression in electroporated banana (Musa spp., cv. 'Bluggoe', ABB group) protoplasts isolated from regenerable embryogenetic cell suspensions.Plant cell reports · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protoplast diameter, a physical parameter controlling the susceptibility to electropermeation, was observed to vary between different batches of Brassica napus and Brassica campestris protoplasts. In order to control the permeation field strength associated with the protoplast size, we investigated the mathematical relationship between permeant field strength and protoplast size by measuring electroloading of the calcein dye under conditions that did not alter protoplast survival. A roughly linear relationship was obtained when the permeant field strength was plotted against the reciprocal of the radius. The plot characteristics were found to be tissue specific (mesophyll, hypocotyl and cotyledon), species specific (turnip, rapeseed) and modulated by the pulse duration. These plots were used as reference curves to determine accurate electrical conditions for DNA transfer whatever the size distributions of different protoplast batches.
Identifiers
24221493What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.