ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1991
The use of antimicrotubule herbicides for the production of doubled haploid plants from anther-derived maize callus.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 142 citations in OpenAlex.
- In vitro- and in vivo-based approaches for doubled haploid production in Zea mays L.: challenges and opportunities.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Haploid identification in maize.Frontiers in plant science · 2024Review
- Paclitaxel and Caffeine-Taurine, New Colchicine Alternatives for Chromosomes Doubling in Maize Haploid Breeding.International journal of molecular sciences · 2023Article
- Article
- Haploid induction and its application in maize breeding.Molecular breeding : new strategies in plant improvement · 2021Article
- Methods for Chromosome Doubling.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Breeding Maize Maternal Haploid Inducers.Plants (Basel, Switzerland) · 2020Review
- In vitro androgenesis: spontaneous vs. artificial genome doubling and characterization of regenerants.Plant cell reports · 2020Review
- The Effect of Caffeine and Trifluralin on Chromosome Doubling in Wheat Anther Culture.Plants (Basel, Switzerland) · 2020Article
- Doubled haploid technology for line development in maize: technical advances and prospects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Novel technologies in doubled haploid line development.Plant biotechnology journal · 2017Review
- Improved production of doubled haploids of winter and spring triticale hybrids via combination of colchicine treatments on anthers and regenerated plants.Journal of applied genetics · 2017Article
- Androgenesis, gynogenesis, and parthenogenesis haploids in cucurbit species.Plant cell reports · 2016Review
- Blockage of mitosis in maize root tips using colchicine-alternatives.Protoplasma · 2010Article
- Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis.Chromosoma · 2004Article
- Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos.Plant cell reports · 2003Article
- Production of haploids from anther culture of banana [Musa balbisiana (BB)].Plant cell reports · 2003Article
- Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii).Plant cell reports · 1996Article
- Oryzalin combined with adventitious regeneration for an efficient chromosome doubling of trihaploid kiwifruit.Plant cell reports · 1996Article
- Colchicine-mediated chromosome doubling during anther culture of maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four antimicrotubule herbicides, amiprophosmethyl (APM), pronamide, oryzalin, and trifluralin, were evaluated for their ability to induce chromosome doubling in anther-derived, haploid maize callus. Effects of various herbicide treatments on the growth and regenerative capacity of callus along with the ploidy and seed set of regenerated plants were determined. Flow cytometric analysis was also used to measure changes in ploidy levels of callus cells following treatments. More than 50% of the cells were doubled in chromosome number after the haploid callus was treated with 5 or 10 μ M APM or 10 μ M pronamide for 3 days. A similar proportion of plants regenerated from the treated callus produced seed upon self-pollination. APM and pronamide did not inhibit callus growth at these concentrations and the treated callus retained a high plant regeneration capacity. Oryzalin very effectively induced chromosome doubling, but severely inhibited the growth of regenerable callus and plant regeneration. Trifluralin induced chromosome doubling in a small proportion of cells at lower concentrations (0.5 and 1 μ M), however, at a higher concentration (5 μM) it inhibited callus growth and plant regeneration. The results indicate that APM and pronamide may be useful agents for inducing chromosome doubling of anther-derived maize haploid callus at very low concentrations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.