ArticleNucleic acids research2014
Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- MicroRNA-Mediated Obstruction of Stem-loop Alternative Splicing (MIMOSAS) regulates long-range alternative splicing in Drosophila.Nucleic acids research · 2026Article
- Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus.Scientific reports · 2021Article
- MicroRNA miR-1002 Enhances NMNAT-Mediated Stress Response by Modulating Alternative Splicing.iScience · 2019Article
- Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito,Viruses · 2019Article
- Multiplexed PCR-Free Detection of MicroRNAs in Single Cancer Cells Using a DNA-Barcoded Microtrough Array Chip.Micromachines · 2019Article
- Knockdown of RNA interference pathway genes impacts the fitness of western corn rootworm.Scientific reports · 2018Article
- Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti.PLoS neglected tropical diseases · 2018Article
- Synthetic miRNAs induce dual arboviral-resistance phenotypes in the vector mosquitoCommunications biology · 2018Article
- Pervasive microRNA Duplication in Chelicerates: Insights from the Embryonic microRNA Repertoire of the Spider Parasteatoda tepidariorum.Genome biology and evolution · 2016Article
- Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.Insects · 2015Review
- A deeply conserved, noncanonical miRNA hosted by ribosomal DNA.RNA (New York, N.Y.) · 2015Article
- Role of microRNAs in arbovirus/vector interactions.Viruses · 2014Review
- Towards a molecular basis of oligometastatic disease: potential role of micro-RNAs.Clinical & experimental metastasis · 2014Review
- Argonaute-dependent small RNAs derived from single-stranded, non-structured precursors.Frontiers in genetics · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Although Dicer is essential for general microRNA (miRNA) biogenesis, vertebrate mir-451 is Dicer independent. Instead, its short pre-miRNA hairpin is 'sliced' by Ago2, then 3'-resected into mature miRNAs. Here, we show that Drosophila cells and animals generate functional small RNAs from mir-451-type precursors. However, their bulk maturation arrests as Ago-cleaved pre-miRNAs, which mostly associate with the RNAi effector AGO2. Routing of pre-mir-451 hairpins to the miRNA effector AGO1 was inhibited by Dicer-1 and its partner Loqs. Loss of these miRNA factors promoted association of pre-mir-451 with AGO1, which sliced them and permitted maturation into ∼ 23-26 nt products. The difference was due to the 3' modification of single-stranded species in AGO2 by Hen1 methyltransferase, whose depletion permitted 3' trimming of Ago-cleaved pre-miRNAs in AGO2. Surprisingly, Nibbler, a 3'-5' exoribonuclease that trims 'long' mature miRNAs in AGO1, antagonized miR-451 processing. We used an in vitro reconstitution assay to identify a soluble, EDTA-sensitive activity that resects sliced pre-miRNAs in AGO1 complexes. Finally, we use deep sequencing to show that depletion of dicer-1 increases the diversity of small RNAs in AGO1, including some candidate mir-451-like loci. Altogether, we document unexpected aspects of miRNA biogenesis and Ago sorting, and provide insights into maturation of Argonaute-cleaved miRNA substrates.
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