Trial reportTissue engineering. Part C, Methods2014
Characterization of a human skin equivalent model to study the effects of ultraviolet B radiation on keratinocytes.
Trial report in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 61 citations in OpenAlex.
- Optimization of a 3D Skin Equivalent Incorporating Melanocytes or Melanoma Cells for In Vitro Melanoma Research.Bioengineering (Basel, Switzerland) · 2026Article
- Article
- Quantum Sensing Unravels Antioxidant Efficacy Within PCL/Matrigel Skin Equivalents.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Hydrogel dressings with intrinsic antibiofilm and antioxidative dual functionalities accelerate infected diabetic wound healing.Nature communications · 2024Article
- Comparison of photodamage in non-pigmented and pigmented human skin equivalents exposed to repeated ultraviolet radiation to investigate the role of melanocytes in skin photoprotection.Frontiers in medicine · 2024Article
- Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies.Biomolecules · 2022Article
- Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice.Marine drugs · 2021Article
- Non-Melanoma Skin Cancers: Biological and Clinical Features.International journal of molecular sciences · 2020Review
- [Use of 2D and 3D cell cultures in dermatology].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2020Review
- Antiphotoaging Potential of Extracts of Yin-Tonic Herbal Medicine in Skin Cell and Human Skin Equivalent.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- AnFrontiers in microbiology · 2020Article
- The Role of the Immune System in Cutaneous Squamous Cell Carcinoma.International journal of molecular sciences · 2019Review
- AlloDerm RTU Integration and Clinical Outcomes When Used for Reconstructive Breast Surgery.Plastic and reconstructive surgery. Global open · 2018Article
- Advances in the Biofabrication of 3D SkinFrontiers in bioengineering and biotechnology · 2018Review
- UVB damage onset and progression 24 h post exposure in human-derived skin cells.Toxicology reports · 2017Article
- Article
- Ursolic Acid-Regulated Energy Metabolism-Reliever or Propeller of Ultraviolet-Induced Oxidative Stress and DNA Damage?Proteomes · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The incidences of skin cancers resulting from chronic ultraviolet radiation (UVR) exposure are on the incline in both Australia and globally. Hence, the cellular and molecular pathways that are associated with UVR-induced photocarcinogenesis need to be urgently elucidated, in order to develop more robust preventative and treatment strategies against skin cancers. In vitro investigations into the effects of UVR (in particular, the highly mutagenic UVB wavelength) have, to date, mainly involved the use of cell culture and animal models. However, these models possess biological disparities to native skin, which, to some extent, have limited their relevance to the in vivo situation. To address this, we characterized a three-dimensional, tissue-engineered human skin equivalent (HSE) model (consisting of primary human keratinocytes cultured on a dermal-derived scaffold) as a representation of a more physiologically relevant platform to study keratinocyte responses to UVB. Significantly, we demonstrate that this model retains several important epidermal properties of native skin. Moreover, UVB irradiation of the HSE constructs was shown to induce key markers of photodamage in the HSE keratinocytes, including the formation of cyclobutane pyrimidine dimers, the activation of apoptotic pathways, the accumulation of p53, and the secretion of inflammatory cytokines. Importantly, we also demonstrate that the UVB-exposed HSE constructs retain the capacity for epidermal repair and regeneration after photodamage. Together, our results demonstrate the potential of this skin equivalent model as a tool to study various aspects of the acute responses of human keratinocytes to UVB radiation damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.