ArticleBMC microbiology2013
CRISPR-MVLST subtyping of Salmonella enterica subsp. enterica serovars Typhimurium and Heidelberg and application in identifying outbreak isolates.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed.
- Article
- Antimicrobial resistance and biofilm formation in rarely reportedFrontiers in microbiology · 2025Article
- CRISPR and CRISPR-MVLST reveal conserved spacer distribution and high similarity among Salmonella enterica serovar Infantis genomes from Brazil and other countries.Molecular genetics and genomics : MGG · 2024Article
- Diversity ofMicrobiology spectrum · 2024Article
- Combined Quantification and Deep Serotyping for Salmonella Risk Profiling in Broiler Flocks.Applied and environmental microbiology · 2023Article
- Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella.Microbiology spectrum · 2022Article
- Salmonella enterica Serovar Typhimurium Isolates from Wild Birds in the United States Represent Distinct Lineages Defined by Bird Type.Applied and environmental microbiology · 2022Article
- Large-Scale Phylogenetic Analysis Reveals a New Genetic Clade among Escherichia coli O26 Strains.Microbiology spectrum · 2022Article
- Evidence for common ancestry and microevolution of passerine-adaptedMicrobial genomics · 2022Article
- Evaluation of the use of CRISPR loci for discrimination ofAIMS microbiology · 2022Article
- Clustered Regularly Interspaced Short Palindromic Repeats Genotyping of Multidrug-ResistantFrontiers in microbiology · 2022Article
- Review
- Antibacterial activity and action mode of chlorogenic acid against Salmonella Enteritidis, a foodborne pathogen in chilled fresh chicken.World journal of microbiology & biotechnology · 2020Article
- Utilization of Clustered Regularly Interspaced Short Palindromic Repeats to GenotypeFrontiers in microbiology · 2020Article
- Diversity of CRISPR/Cas system in Clostridium perfringens.Molecular genetics and genomics : MGG · 2019Article
- Laboratory characterisation of Salmonella enterica serotype Typhi isolates from Zimbabwe, 2009-2017.BMC infectious diseases · 2019Article
- Article
- Assessment and Comparison of Molecular Subtyping and Characterization Methods forFrontiers in microbiology · 2019Review
- High-Resolution Identification of Multiple Salmonella Serovars in a Single Sample by Using CRISPR-SeroSeq.Applied and environmental microbiology · 2018Article
- Review
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5 authors.
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Abstract
backgroundSalmonella enterica subsp. enterica serovars Typhimurium (S. Typhimurium) and Heidelberg (S. Heidelberg) are major causes of foodborne salmonellosis, accounting for a fifth of all annual salmonellosis cases in the United States. Rapid, efficient and accurate methods for identification are required for routine surveillance and to track specific strains during outbreaks. We used Pulsed-field Gel Electrophoresis (PFGE) and a recently developed molecular subtyping approach termed CRISPR-MVLST that exploits the hypervariable nature of virulence genes and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) to subtype clinical S. Typhimurium and S. Heidelberg isolates.
resultsWe analyzed a broad set of 175 S. Heidelberg and S. Typhimurium isolates collected over a five-year period. We identified 21 Heidelberg Sequence Types (HSTs) and 37 Typhimurium STs (TSTs) that were represented by 27 and 45 PFGE pulsotypes, respectively, and determined the discriminatory power of each method.
conclusionsFor S. Heidelberg, our data shows that combined typing by both CRISPR-MVLST and PFGE provided a discriminatory power of 0.9213. Importantly, CRISPR-MVLST was able to separate common PFGE patterns such as JF6X01.0022 into distinct STs, thus providing significantly greater discriminatory power. Conversely, we show that subtyping by either CRISPR-MVLST or PFGE independently provides a sufficient discriminatory power (0.9345 and 0.9456, respectively) for S. Typhimurium. Additionally, using isolates from two S. Typhimurium outbreaks, we demonstrate that CRISPR-MVLST provides excellent epidemiologic concordance.
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