ArticleMicrobial cell factories2013
Development of a broad-host synthetic biology toolbox for Ralstonia eutropha and its application to engineering hydrocarbon biofuel production.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Cas3-mediated genome reduction: demonstration in Cupriavidus necator H16 improves growth on heterotrophic and autotrophic carbon sources.Nucleic acids research · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Physiology-informed use of Cupriavidus necator in biomanufacturing: a review of advances and challenges.Microbial cell factories · 2025Review
- From Knallgas Bacterium to Promising Biomanufacturing Host: The Evolution of Cupriavidus necator.Advances in biochemical engineering/biotechnology · 2025Review
- Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide.World journal of microbiology & biotechnology · 2024Review
- Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator).Applied microbiology and biotechnology · 2024Review
- Controlling the expression of heterologous genes in Bdellovibrio bacteriovorus using synthetic biology strategies.Microbial biotechnology · 2024Article
- Techno-Economic Assessment of Electromicrobial Production ofEnvironmental science & technology · 2024Article
- Toolbox of Characterized Genetic Parts forACS synthetic biology · 2024Article
- Expanding the synthetic biology toolbox of Cupriavidus necator for establishing fatty acid production.Journal of industrial microbiology & biotechnology · 2024Article
- Perspectives for Using COBioengineering (Basel, Switzerland) · 2023Review
- Autotrophic Production of the Sesquiterpene α-Humulene withBioengineering (Basel, Switzerland) · 2023Article
- The pGinger Family of Expression Plasmids.Microbiology spectrum · 2023Article
- Engineering osmolysis susceptibility in Cupriavidus necator and Escherichia coli for recovery of intracellular products.Microbial cell factories · 2023Article
- Trends in Research and Development for COACS omega · 2023Review
- Conversion of COProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant BacteriumACS synthetic biology · 2022Article
- Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from COMicrobial cell factories · 2022Article
- Adaptive evolution of Methylotuvimicrobium alcaliphilum to grow in the presence of rhamnolipids improves fatty acid and rhamnolipid production from CH4.Journal of industrial microbiology & biotechnology · 2022Article
- Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.Applied and environmental microbiology · 2022Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe chemoautotrophic bacterium Ralstonia eutropha can utilize H2/CO2 for growth under aerobic conditions. While this microbial host has great potential to be engineered to produce desired compounds (beyond polyhydroxybutyrate) directly from CO2, little work has been done to develop genetic part libraries to enable such endeavors.
resultsWe report the development of a toolbox for the metabolic engineering of Ralstonia eutropha H16. We have constructed a set of broad-host-range plasmids bearing a variety of origins of replication, promoters, 5' mRNA stem-loop structures, and ribosomal binding sites. Specifically, we analyzed the origins of replication pCM62 (IncP), pBBR1, pKT (IncQ), and their variants. We tested the promoters P(BAD), T7, P(xyls/PM), P(lacUV5), and variants thereof for inducible expression. We also evaluated a T7 mRNA stem-loop structure sequence and compared a set of ribosomal binding site (RBS) sequences derived from Escherichia coli, R. eutropha, and a computational RBS design tool. Finally, we employed the toolbox to optimize hydrocarbon production in R. eutropha and demonstrated a 6-fold titer improvement using the appropriate combination of parts.
conclusionWe constructed and evaluated a versatile synthetic biology toolbox for Ralstonia eutropha metabolic engineering that could apply to other microbial hosts as well.
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