ArticleBMC medical imaging2013
The use of high-frequency ultrasound imaging and biofluorescence for in vivo evaluation of gene therapy vectors.
Article in BMC medical imaging, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- A Single Short 'Tone Burst' Results in Optimal Drug Delivery to Tumours Using Ultrasound-Triggered Therapeutic Microbubbles.Pharmaceutics · 2022Article
- Affimer Tagged Cubosomes: Targeting of Carcinoembryonic Antigen Expressing Colorectal Cancer Cells UsingACS applied materials & interfaces · 2022Article
- Ultrasonic technologies in imaging and drug delivery.Cellular and molecular life sciences : CMLS · 2021Review
- Development of orthotopic tumour models using ultrasound-guided intrahepatic injection.Scientific reports · 2019Article
- Assessment of Venous Thrombosis in Animal Models.Arteriosclerosis, thrombosis, and vascular biology · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNon-invasive imaging of the biodistribution of novel therapeutics including gene therapy vectors in animal models is essential.
methodsThis study assessed the utility of high-frequency ultrasound (HF-US) combined with biofluoresence imaging (BFI) to determine the longitudinal impact of a Herpesvirus saimiri amplicon on human colorectal cancer xenograft growth.
resultsHF-US imaging of xenografts resulted in an accurate and informative xenograft volume in a longitudinal study. The volumes correlated better with final ex vivo volume than mechanical callipers (R2 = 0.7993, p = 0.0002 vs. R2 = 0.7867, p = 0.0014). HF-US showed that the amplicon caused lobe formation. BFI demonstrated retention and expression of the amplicon in the xenografts and quantitation of the fluorescence levels also correlated with tumour volumes.
conclusionsThe use of multi-modal imaging provided useful and enhanced insights into the behaviour of gene therapy vectors in vivo in real-time. These relatively inexpensive technologies are easy to incorporate into pre-clinical studies.
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Registered trials
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