ArticleProceedings of the National Academy of Sciences of the United States of America2013
Global methylation state at base-pair resolution of the Caulobacter genome throughout the cell cycle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 117 citations in OpenAlex.
- Genome replication and cell division control by the methylation-sensitive GcrA regulator in Agrobacterium tumefaciens.Nucleic acids research · 2026Article
- Epigenetic Phase Variation in Bacterial Adaptation to Environmental Stress: A Narrative Review.Current microbiology · 2026Review
- Cytosine methylation contributes to the fitness ofiScience · 2026Article
- Article
- DNA methylation by CcrM contributes to genome maintenance in the Agrobacterium tumefaciens plant pathogen.Nucleic acids research · 2024Article
- Comparison of CcrM-dependent methylation inJournal of bacteriology · 2024Article
- Article
- Comparison of CcrM-dependent methylation inbioRxiv : the preprint server for biology · 2024Article
- Article
- Comparison ofEpigenomes · 2023Article
- Bacterial DNA methyltransferase: A key to the epigenetic world with lessons learned from proteobacteria.Frontiers in microbiology · 2023Review
- Means, mechanisms and consequences of adenine methylation in DNA.Nature reviews. Genetics · 2022Review
- Precision Methylome and In Vivo Methylation Kinetics Characterization of Klebsiella pneumoniae.Genomics, proteomics & bioinformatics · 2022Article
- Diverse DNA modification in marine prokaryotic and viral communities.Nucleic acids research · 2022Article
- DNA Methylation inmSystems · 2022Article
- DNA Methylation in Prokaryotes.Advances in experimental medicine and biology · 2022Article
- Advanced biotechnology using methyltransferase and its applications in bacteria: a mini review.Biotechnology letters · 2022Review
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- Improving Mobilization of Foreign DNA into Zymomonas mobilis Strain ZM4 by Removal of Multiple Restriction Systems.Applied and environmental microbiology · 2021Article
- DNA methylation by three Type I restriction modification systems of Escherichia coli does not influence gene regulation of the host bacterium.Nucleic acids research · 2021Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
The Caulobacter DNA methyltransferase CcrM is one of five master cell-cycle regulators. CcrM is transiently present near the end of DNA replication when it rapidly methylates the adenine in hemimethylated GANTC sequences. The timing of transcription of two master regulator genes and two cell division genes is controlled by the methylation state of GANTC sites in their promoters. To explore the global extent of this regulatory mechanism, we determined the methylation state of the entire chromosome at every base pair at five time points in the cell cycle using single-molecule, real-time sequencing. The methylation state of 4,515 GANTC sites, preferentially positioned in intergenic regions, changed progressively from full to hemimethylation as the replication forks advanced. However, 27 GANTC sites remained unmethylated throughout the cell cycle, suggesting that these protected sites could participate in epigenetic regulatory functions. An analysis of the time of activation of every cell-cycle regulatory transcription start site, coupled to both the position of a GANTC site in their promoter regions and the time in the cell cycle when the GANTC site transitions from full to hemimethylation, allowed the identification of 59 genes as candidates for epigenetic regulation. In addition, we identified two previously unidentified N(6)-methyladenine motifs and showed that they maintained a constant methylation state throughout the cell cycle. The cognate methyltransferase was identified for one of these motifs as well as for one of two 5-methylcytosine motifs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.