ArticleJournal of experimental botany2014
TRV-GFP: a modified Tobacco rattle virus vector for efficient and visualizable analysis of gene function.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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74 citing papers in PubMed, 168 citations in OpenAlex.
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- The RhMPK3-RhLOB41-RhWRKY9 module orchestrates ethylene-induced petal abscission via dual control of ROS homeostasis in rose.Science advances · 2026Article
- Establishment of a TRV-VIGS system in Coix (Frontiers in plant science · 2026Article
- RD21 enhances resistance to the strawberry vein banding virus by promoting autophagy-mediated degradation of the viral silencing suppressor P6 protein.Plant biotechnology journal · 2025Article
- Establishment and optimization of a tobacco rattle virus -based virus-induced gene Silencing in Atriplex canescens.Plant methods · 2025Article
- The GAs-RhMYB70 feedback loop fine-tunes cell expansion and petal size by modulating cellulose content in rose.Horticulture research · 2025Article
- RcPLATZ8 as a novel negative regulator of flowering in Rosa chinensis.Plant cell reports · 2025Article
- Efficient Virus-Induced Gene Silencing (VIGS) Method for Discovery of Resistance Genes in Soybean.Plants (Basel, Switzerland) · 2025Article
- RcSRR1 interferes with the RcCSN5B-mediated deneddylation of RcCRL4 to modulate RcCO proteolysis and prevent rose flowering under red light.Horticulture research · 2025Article
- Characterization and Functional Analysis of RhHsfA7, a Heat Stress Transcription Factor in Roses (Plants (Basel, Switzerland) · 2025Article
- The NbCBP1-NbSAMS1 Module Promotes Ethylene Accumulation to EnhanceInternational journal of molecular sciences · 2025Article
- The MADS-Box Transcription Factor CaRIN Positively Regulates Chlorophyll Degradation During Pepper (Plants (Basel, Switzerland) · 2025Article
- The Application of Virus-Induced Gene Silencing in the Study of Rose Petal Abscission.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Virus-Induced Gene Silencing (VIGS) inPlants (Basel, Switzerland) · 2024Article
- Trans-complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector.Plant biotechnology journal · 2024Article
- The transcription factor RhMYB17 regulates the homeotic transformation of floral organs in rose (Rosa hybrida) under cold stress.Journal of experimental botany · 2024Article
- The F-box protein RhSAF destabilizes the gibberellic acid receptor RhGID1 to mediate ethylene-induced petal senescence in rose.The Plant cell · 2024Article
- Establishment of a Homologous Silencing System with Intact-Plant Infiltration and Minimized Operation for Studying Gene Function in Herbaceous Peonies.International journal of molecular sciences · 2024Article
- TSPO-induced degradation of the ethylene receptor RhETR3 promotes salt tolerance in rose (Horticulture research · 2024Article
- The cellular and molecular basis of the spur development inHorticulture research · 2024Article
14 more citing papers are in PubMed but not listed here.
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10 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Virus-induced gene silencing (VIGS) is a useful tool for functional characterization of genes in plants. Unfortunately, the efficiency of infection by Tobacco rattle virus (TRV) is relatively low for some non-Solanaceae plants, which are economically important, such as rose (Rosa sp.). Here, to generate an easy traceable TRV vector, a green fluorescent protein (GFP) gene was tagged to the 3' terminus of the coat protein gene in the original TRV2 vector, and the silencing efficiency of the modified TRV-GFP vector was tested in several plants, including Nicotiana benthamiana, Arabidopsis thaliana, rose, strawberry (Fragaria ananassa), and chrysanthemum (Dendranthema grandiflorum). The results showed that the efficiency of infection by TRV-GFP was equal to that of the original TRV vector in each tested plant. Spread of the modified TRV virus was easy to monitor by using fluorescent microscopy and a hand-held UV lamp. When TRV-GFP was used to silence the endogenous phytoene desaturase (PDS) gene in rose cuttings and seedlings, the typical photobleached phenotype was observed in 75-80% plants which were identified as GFP positive by UV lamp. In addition, the abundance of GFP protein, which represented the concentration of TRV virus, was proved to correlate negatively with the level of the PDS gene, suggesting that GFP could be used as an indicator of the degree of silencing of a target gene. Taken together, this work provides a visualizable and efficient tool to predict positive gene silencing plants, which is valuable for research into gene function in plants, especially for non-Solanaceae plants.
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