ArticlePlanta2014
Functional identification of multiple nucleocytoplasmic trafficking signals in the broad-spectrum resistance protein RPW8.2.
Article in Planta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
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- Identification of HSF gene family and functional analysis of two HSFA1 genes with tandem repeat features in Fritillaria cirrhosa D.Don.BMC genomics · 2026Article
- Genome-wide characterization of theFrontiers in plant science · 2025Article
- Integrative analysis of the metabolome and transcriptome provides insights into the mechanisms of lignan biosynthesis in Herpetospermum pedunculosum (Cucurbitaceae).BMC genomics · 2024Article
- Magnaporthe oryzae effector MoSPAB1 directly activates rice Bsr-d1 expression to facilitate pathogenesis.Nature communications · 2023Article
- Insights into ZmWAKL in maize kernel development: genome-wide investigation and GA-mediated transcription.BMC genomics · 2023Article
- Functional Analysis of Bna-miR399c-Life (Basel, Switzerland) · 2023Article
- High-Resolution Crystal Structure of Muscle Phosphoglycerate Mutase Provides Insight into Its Nuclear Import and Role.International journal of molecular sciences · 2022Article
- Osa-miR535 targets SQUAMOSA promoter binding protein-like 4 to regulate blast disease resistance in rice.The Plant journal : for cell and molecular biology · 2022Article
- A Novel, Small Cysteine-Rich Effector, RsSCR10 inFrontiers in microbiology · 2021Article
- The Flower-Infecting FungusFrontiers in plant science · 2021Article
- Heterologous Expression of Dehydration-InducibleInternational journal of molecular sciences · 2020Article
- TheGenes · 2019Article
- RESISTANCE TO POWDERY MILDEW8.1 boosts pattern-triggered immunity against multiple pathogens in Arabidopsis and rice.Plant biotechnology journal · 2018Article
- miR396-Frontiers in plant science · 2018Article
- Article
- Osa-miR169 Negatively Regulates Rice Immunity against the Blast FungusFrontiers in plant science · 2017Article
- Biotrophy at Its Best: Novel Findings and Unsolved Mysteries of the Arabidopsis-Powdery Mildew Pathosystem.The arabidopsis book · 2016Article
- Article
- Cloning and functional validation of molybdenum cofactor sulfurase gene from Ammopiptanthus nanus.Plant cell reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear localization signals (NLSs) and nuclear export signals (NESs) are important intramolecular regulatory elements for protein nucleocytoplasmic trafficking. This regulation confers spatial specificity to signal initiation and transduction in eukaryotic cells and thus is fundamental to the viability of all eukaryotic organisms. Here, we developed a simple and rapid method in which activity of putative NLSs or NESs was reported by subcellular localization of two tandem fluorescent proteins in fusion with the respective NLSs or NESs after agroinfiltration-mediated transient expression in leaves of Nicotiana benthamiana (Nb). We further demonstrated that the predicted NES from amino acid residue (aa) 9 to 22 and the NLS from aa91 to 101 in the broad-spectrum disease resistance protein RPW8.2 possess nuclear export and import activity, respectively. Additionally, by testing overlapping fragments covering the full length of RPW8.2, we identified another NLS from aa65 to 74 with strong nuclear import activity and two tandem non-canonical NESs in the C-terminus with strong nuclear export activity. Taken together, our results demonstrated the utility of a simple method to evaluate potential NLSs and NESs in plant cells and suggested that RPW8.2 may be subject to opposing nucleocytoplasmic trafficking forces for its subcellular localization and functional execution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.