ArticleMolecular biology and evolution2014
Differential expression of conserved germ line markers and delayed segregation of male and female primordial germ cells in a hermaphrodite, the leech helobdella.
Article in Molecular biology and evolution, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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15 citing papers in PubMed, 38 citations in OpenAlex.
- Faster and smaller: Heterochronic shift in gene expression leads to larval onset of spermatogenesis and miniaturized males.iScience · 2026Article
- Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis.The Journal of experimental biology · 2024Article
- Acceleration of genome rearrangement in clitellate annelids.bioRxiv : the preprint server for biology · 2024Article
- Germ Line/Multipotency Genes Show Differential Expression during Embryonic Development of the AnnelidBiology · 2023Article
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- Spatiotemporal Expression of Anticoagulation FactorInternational journal of molecular sciences · 2019Article
- Identification and expression pattern of a new digestive invertebrate-type lysozyme from the earthworm.Genes & genomics · 2019Article
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- Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelideLife · 2017Article
- Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis.RNA biology · 2017Article
- Integrated gene mapping and synteny studies give insights into the evolution of a sex proto-chromosome in Solea senegalensis.Chromosoma · 2017Article
- On the role of the proventricle region in reproduction and regeneration in Typosyllis antoni (Annelida: Syllidae).BMC evolutionary biology · 2016Article
- Developmental expression of "germline"- and "sex determination"-related genes in the ctenophore Mnemiopsis leidyi.EvoDevo · 2016Article
- Leeches of the genus Helobdella as model organisms for Evo-Devo studies.Theory in biosciences = Theorie in den Biowissenschaften · 2015Article
- Developmental biology of the leech Helobdella.The International journal of developmental biology · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
In sexually reproducing animals, primordial germ cells (PGCs) are often set aside early in embryogenesis, a strategy that minimizes the risk of genomic damage associated with replication and mitosis during the cell cycle. Here, we have used germ line markers (piwi, vasa, and nanos) and microinjected cell lineage tracers to show that PGC specification in the leech genus Helobdella follows a different scenario: in this hermaphrodite, the male and female PGCs segregate from somatic lineages only after more than 20 rounds of zygotic mitosis; the male and female PGCs share the same (mesodermal) cell lineage for 19 rounds of zygotic mitosis. Moreover, while all three markers are expressed in both male and female reproductive tissues of the adult, they are expressed differentially between the male and female PGCs of the developing embryo: piwi and vasa are expressed preferentially in female PGCs at a time when nanos is expressed preferentially in male PGCs. A priori, the delayed segregation of male and female PGCs from somatic tissues and from one another increases the probability of mutations affecting both male and female PGCs of a given individual. We speculate that this suite of features, combined with a capacity for self-fertilization, may contribute to the dramatically rearranged genome of Helobdella robusta relative to other animals.
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