ArticleThe Journal of antimicrobial chemotherapy2014
Novel mutations in penicillin-binding protein genes in clinical Staphylococcus aureus isolates that are methicillin resistant on susceptibility testing, but lack the mec gene.
Article in The Journal of antimicrobial chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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Who cites it
46 citing papers in PubMed, 123 citations in OpenAlex.
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- The Spx stress regulator confers high-level β-lactam resistance and decreases susceptibility to last-line antibiotics in methicillin-resistantAntimicrobial agents and chemotherapy · 2024Article
- Phenotypic and genotypic characterization of linezolid resistance and the effect of antibiotic combinations on methicillin-resistant Staphylococcus aureus clinical isolates.Annals of clinical microbiology and antimicrobials · 2023Article
- Biofilm forming multidrug resistantIranian journal of veterinary research · 2023Article
- Evaluation of the Antibacterial Activity of Quinoxaline Derivative Compound Against Methicillin-ResistantInfection and drug resistance · 2023Article
- Shared antibiotic resistance and virulence genes in Staphylococcus aureus from diverse animal hosts.Scientific reports · 2022Article
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- Accessory Genome Dynamics of Local and GlobalFrontiers in microbiology · 2022Article
- Modified methicillin-resistant Staphylococcus aureus detected in neonatal intensive care patients.The Journal of antimicrobial chemotherapy · 2021Article
- Mutations in theMicrobial genomics · 2021Article
- Antimicrobial Resistance and Genetic Lineages ofAnimals : an open access journal from MDPI · 2021Article
- Multidrug-resistant Staphylococcus cohnii and Staphylococcus urealyticus isolates from German dairy farms exhibit resistance to beta-lactam antibiotics and divergent penicillin-binding proteins.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
objectivesMethicillin-resistant Staphylococcus aureus (MRSA) is an important global health problem. MRSA resistance to β-lactam antibiotics is mediated by the mecA or mecC genes, which encode an alternative penicillin-binding protein (PBP) 2a that has a low affinity to β-lactam antibiotics. Detection of mec genes or PBP2a is regarded as the gold standard for the diagnosis of MRSA. We identified four MRSA isolates that lacked mecA or mecC genes, but were still phenotypically resistant to pencillinase-resistant β-lactam antibiotics.
methodsThe four human S. aureus isolates were investigated by whole genome sequencing and a range of phenotypic assays.
resultsWe identified a number of amino acid substitutions present in the endogenous PBPs 1, 2 and 3 that were found in the resistant isolates but were absent in closely related susceptible isolates and which may be the basis of resistance. Of particular interest are three identical amino acid substitutions in PBPs 1, 2 and 3, occurring independently in isolates from at least two separate multilocus sequence types. Two different non-conservative substitutions were also present in the same amino acid of PBP1 in two isolates from two different sequence types.
conclusionsThis work suggests that phenotypically resistant MRSA could be misdiagnosed using molecular methods alone and provides evidence of alternative mechanisms for β-lactam resistance in MRSA that may need to be considered by diagnostic laboratories.
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