ArticleAnalytical biochemistry2014
Weak protein-protein interactions revealed by immiscible filtration assisted by surface tension.
Article in Analytical biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Extraction of redox extracellular vesicles using exclusion-based sample preparation.Analytical and bioanalytical chemistry · 2024Article
- High-Efficiency Enrichment by Saturating Nanoliters of Protein Affinity Media.Analytical chemistry · 2023Article
- On the utility of microfluidic systems to study protein interactions: advantages, challenges, and applications.European biophysics journal : EBJ · 2023Review
- Improving wastewater-based epidemiology performance through streamlined automation.Journal of environmental chemical engineering · 2023Article
- Development and Validation of a Simplified Method for Analysis of SARS-CoV-2 RNA in University Dormitories.ACS ES&T water · 2022Article
- Analytical validation and initial clinical testing of quantitative microscopic evaluation for PD-L1 and HLA I expression on circulating tumor cells from patients with non-small cell lung cancer.Biomarker research · 2022Article
- Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma.Molecular oncology · 2021Article
- Oil immersed lossless total analysis system for integrated RNA extraction and detection of SARS-CoV-2.Nature communications · 2021Article
- Centrifugation-Assisted Immiscible Fluid Filtration for Dual-Bioanalyte Extraction.Analytical chemistry · 2019Article
- Magnetic System for Automated Manipulation of Paramagnetic Particles.Analytical chemistry · 2016Article
- AirJump: Using Interfaces to Instantly Perform Simultaneous Extractions.ACS applied materials & interfaces · 2016Article
- Efficient sample preparation from complex biological samples using a sliding lid for immobilized droplet extractions.Analytical chemistry · 2014Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Biological mechanisms are often mediated by transient interactions between multiple proteins. The isolation of intact protein complexes is essential to understanding biochemical processes and an important prerequisite for identifying new drug targets and biomarkers. However, low-affinity interactions are often difficult to detect. Here, we use a newly described method called immiscible filtration assisted by surface tension (IFAST) to isolate proteins under defined binding conditions. This method, which gives a near-instantaneous isolation, enables significantly higher recovery of transient complexes compared to current wash-based protocols, which require reequilibration at each of several wash steps, resulting in protein loss. The method moves proteins, or protein complexes, captured on a solid phase through one or more immiscible-phase barriers that efficiently exclude the passage of nonspecific material in a single operation. We use a previously described polyol-responsive monoclonal antibody to investigate the potential of this new method to study protein binding. In addition, difficult-to-isolate complexes involving the biologically and clinically important Wnt signaling pathway were isolated. We anticipate that this simple, rapid method to isolate intact, transient complexes will enable the discoveries of new signaling pathways, biomarkers, and drug targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.