ArticleBMC genomics2013
Urinary proteomic and non-prefractionation quantitative phosphoproteomic analysis during pregnancy and non-pregnancy.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Proteomic changes induced by acute exercise and high-intensity interval training during pregnancy: a pilot randomised controlled trial.Scientific reports · 2025Trial
- Exploratory Urinary Proteomic Profiling in Pregnancies with Fetal Aneuploidies: Molecular Insights into Maternal-Fetal Metabolic Communication.Current issues in molecular biology · 2025Article
- Recent progress in mass spectrometry-based urinary proteomics.Clinical proteomics · 2024Review
- Profiling Phosphoproteome Landscape in Circulating Extracellular Vesicles from Microliters of Biofluids through Functionally Tunable Paramagnetic Separation.Angewandte Chemie (International ed. in English) · 2023Article
- Osmoregulatory strategies of estuarine fish Scatophagus argus in response to environmental salinity changes.BMC genomics · 2022Article
- Urinary proteomic analysis during pregnancy and its potential application in early prediction of gestational diabetes mellitus and spontaneous abortion.Annals of translational medicine · 2022Article
- Identification of Structural and Molecular Signatures Mediating Adaptive Changes in the Mouse Kidney in Response to Pregnancy.International journal of molecular sciences · 2022Article
- HBFP: a new repository for human body fluid proteome.Database : the journal of biological databases and curation · 2021Article
- Human body-fluid proteome: quantitative profiling and computational prediction.Briefings in bioinformatics · 2021Review
- Comparative phosphoproteomic analysis of BR-defective mutant reveals a key role of GhSK13 in regulating cotton fiber development.Science China. Life sciences · 2020Article
- Within-individual and interlaboratory variability analyses of urinary metabolites measurements of organophosphorus insecticides.Journal of exposure science & environmental epidemiology · 2020Article
- Bioinformatics for Renal and Urinary Proteomics: Call for Aggrandization.International journal of molecular sciences · 2020Review
- Comprehensive Identification and Characterization of Human Secretome Based on Integrative Proteomic and Transcriptomic Data.Frontiers in cell and developmental biology · 2019Article
- Article
- Clinically Relevant Post-Translational Modification Analyses-Maturing Workflows and Bioinformatics Tools.International journal of molecular sciences · 2018Review
- Highly Efficient Phosphoproteome Capture and Analysis from Urinary Extracellular Vesicles.Journal of proteome research · 2018Article
- Fast quantitative urinary proteomic profiling workflow for biomarker discovery in kidney cancer.Clinical proteomics · 2018Article
- A comprehensive analysis and annotation of human normal urinary proteome.Scientific reports · 2017Article
- Phosphoproteome Discovery in Human Biological Fluids.Proteomes · 2016Review
- Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation.Clinical proteomics · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProgress in the fields of protein separation and identification technologies has accelerated research into biofluids proteomics for protein biomarker discovery. Urine has become an ideal and rich source of biomarkers in clinical proteomics. Here we performed a proteomic analysis of urine samples from pregnant and non-pregnant patients using gel electrophoresis and high-resolution mass spectrometry. Furthermore, we also apply a non-prefractionation quantitative phosphoproteomic approach using mTRAQ labeling to evaluate the expression of specific phosphoproteins during pregnancy comparison with non-pregnancy.
resultsIn total, 2579 proteins (10429 unique peptides) were identified, including 1408 from the urine of pregnant volunteers and 1985 from the urine of non-pregnant volunteers. One thousand and twenty-three proteins were not reported in previous studies at the proteome level and were unique to our study. Furthermore, we obtained 237 phosphopeptides, representing 105 phosphoproteins. Among these phosphoproteins, 16 of them were found to be significantly differentially expressed, of which 14 were up-regulated and two were down-regulated in urine samples from women just before vaginal delivery.
conclusionTaken together, these results offer a comprehensive urinary proteomic profile of healthy women during before and after vaginal delivery and novel information on the phosphoproteins that are differentially regulated during the maintenance of normal pregnancy. Our results may provide a better understanding of the mechanisms of pregnancy maintenance, potentially leading to the development of biomarker-based sensitive assays for understanding pregnancy.
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