ArticleBioTechniques2013
New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe.
Article in BioTechniques, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Evolutionarily Conserved but Mechanistically Distinct Mitochondrial Responses to Loss of Timeless/Swi1.Biomolecules · 2026Article
- A role for condensin-mediator interaction in mitotic chromosome organization.Nature communications · 2026Article
- Analysis of the mitochondria-associated degradation pathway (MAD) in yeast cells.Methods in enzymology · 2024Article
- ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.Nucleic acids research · 2023Article
- Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.PLoS genetics · 2022Article
- Ccq1-Raf2 interaction mediates CLRC recruitment to establish heterochromatin at telomeres.Life science alliance · 2021Article
- Mitochondria-Associated Degradation Pathway (MAD) Function beyond the Outer Membrane.Cell reports · 2020Article
- Evolution of Substrates and Components of the Pro/N-Degron Pathway.Biochemistry · 2020Article
- Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins.Microbial cell factories · 2019Article
- Architectural alterations of the fission yeast genome during the cell cycle.Nature structural & molecular biology · 2017Article
- Single-step Marker Switching inBio-protocol · 2016Article
- Transcription factors mediate condensin recruitment and global chromosomal organization in fission yeast.Nature genetics · 2016Article
- Swi1Timeless Prevents Repeat Instability at Fission Yeast Telomeres.PLoS genetics · 2016Article
- Interaction of the Jhd2 Histone H3 Lys-4 Demethylase with Chromatin Is Controlled by Histone H2A Surfaces and Restricted by H2B Ubiquitination.The Journal of biological chemistry · 2015Article
- Chromatin immunoprecipitation to detect DNA replication and repair factors.Methods in molecular biology (Clifton, N.J.) · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We describe a series of new vectors for PCR-based epitope tagging and gene disruption in the fission yeast Schizosaccharomyces pombe, an exceptional model organism for the study of cellular processes. The vectors are designed for amplification of gene-targeting DNA cassettes and integration into specific genetic loci, allowing expression of proteins fused to 12 tandem copies of the Pk (V5) epitope or 5 tandem copies of the FLAG epitope with a glycine linker. These vectors are available with various antibiotic or nutritional markers and are useful for protein studies using biochemical and cell biological methods. We also describe new vectors for fluorescent protein-tagging and gene disruption using ura4MX6, LEU2MX6, and his3MX6 selection markers, allowing researchers in the S. pombe community to disrupt genes and manipulate genomic loci using primer sets already available for the widely used pFA6a-MX6 system. Our new vectors may also be useful for gene manipulation in Saccharomyces cerevisiae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.