ArticleJournal of bacteriology2014
Modulation of the Bacillus anthracis secretome by the immune inhibitor A1 protease.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- The Dual Role of RsiP in Regulating Virulence and Host Adaptation inPathogens (Basel, Switzerland) · 2026Article
- Bacterial zinc metalloproteases as facilitators of pathogenic success: a review.Archives of microbiology · 2026Review
- First Insight into the Degradome ofInternational journal of molecular sciences · 2024Article
- Characterization of tryptanthrin as an antibacterial reagent inhibiting Vibrio splendidus.Applied microbiology and biotechnology · 2024Article
- Quantifying in vitro B. anthracis growth and PA production and decay: a mathematical modelling approach.NPJ systems biology and applications · 2024Article
- Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-PathogenicAntibiotics (Basel, Switzerland) · 2024Article
- BrnQ-Type Branched-Chain Amino Acid Transporters Influence Bacillus anthracis Growth and Virulence.mBio · 2022Article
- Production of aLife (Basel, Switzerland) · 2022Article
- Immune Inhibitor A Metalloproteases Contribute to Virulence inInfection and immunity · 2021Article
- AtxA-Controlled Small RNAs ofFrontiers in microbiology · 2020Article
- TheMicrobiology spectrum · 2019Review
- Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach.Journal of proteome research · 2018Article
- A Dual Role for theFrontiers in microbiology · 2018Article
- InhA1-Mediated Cleavage of the Metalloprotease NprA AllowsFrontiers in microbiology · 2018Article
- Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions.BMC genomics · 2016Article
- Article
- Structural Basis for Latency and Function of Immune Inhibitor A Metallopeptidase, a Modulator of the Bacillus anthracis Secretome.Structure (London, England : 1993) · 2016Article
- Bacillus anthracis Overcomes an Amino Acid Auxotrophy by Cleaving Host Serum Proteins.Journal of bacteriology · 2015Article
- Proteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors.Frontiers in microbiology · 2015Article
- Whole genome protein microarrays for serum profiling of immunodominant antigens of Bacillus anthracis.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The Bacillus anthracis secretome includes protective antigen, lethal factor, and edema factor, which are the components of anthrax toxin, and other proteins with known or potential roles in anthrax disease. Immune inhibitor A1 (InhA1) is a secreted metalloprotease that is unique to pathogenic members of the Bacillus genus and has been associated with cleavage of host proteins during infection. Here, we report the effect of InhA1 on the B. anthracis secretome. Differential in-gel electrophoresis of proteins present in culture supernatants from a parent strain and an isogenic inhA1-null mutant revealed multiple differences. Of the 1,340 protein spots observed, approximately one-third were less abundant and one-third were more abundant in the inhA1 secretome than in the parent strain secretome. Proteases were strongly represented among those proteins exhibiting a 9-fold or greater change. InhA1 purified from a B. anthracis culture supernatant directly cleaved each of the anthrax toxin proteins as well as an additional secreted protease, Npr599. The conserved zinc binding motif HEXXH of InhA1 (HEYGH) was critical for its proteolytic activity. Our data reveal that InhA1 directly and indirectly modulates the form and/or abundance of over half of all the secreted proteins of B. anthracis. The proteolytic activity of InhA1 on established secreted virulence factors, additional proteases, and other secreted proteins suggests that this major protease plays an important role in virulence not only by cleaving mammalian substrates but also by modulating the B. anthracis secretome itself.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.