ArticleJournal of bacteriology2014
Geranylgeranyl reductase and ferredoxin from Methanosarcina acetivorans are required for the synthesis of fully reduced archaeal membrane lipid in Escherichia coli cells.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 48 citations in OpenAlex.
- Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.Applied microbiology and biotechnology · 2026Article
- NapR, a novel nucleoid-associated protein, regulates antioxidant defense in mycobacteria.mSphere · 2025Article
- A dynamic protein interactome drives energy conservation and electron flux inApplied and environmental microbiology · 2025Article
- Article
- Optimizing Archaeal Lipid Biosynthesis inACS synthetic biology · 2024Article
- The catalytic and structural basis of archaeal glycerophospholipid biosynthesis.Extremophiles : life under extreme conditions · 2022Review
- Identification of a protein responsible for the synthesis of archaeal membrane-spanning GDGT lipids.Nature communications · 2022Article
- Engineering the bilayer: Emerging genetic tool kits for mechanistic lipid biology.Current opinion in chemical biology · 2021Review
- Bridging the membrane lipid divide: bacteria of the FCB group superphylum have the potential to synthesize archaeal ether lipids.The ISME journal · 2021Article
- An automated workflow to screen alkene reductases using high-throughput thin layer chromatography.Biotechnology for biofuels · 2020Article
- CbrA Mediates Colicin M Resistance in Escherichia coli through Modification of Undecaprenyl-Phosphate-Linked Peptidoglycan Precursors.Journal of bacteriology · 2020Article
- Construction of an artificial biosynthetic pathway for hyperextended archaeal membrane lipids in the bacteriumSynthetic biology (Oxford, England) · 2020Article
- Isoprenoids enhance the stability of fatty acid membranes at the emergence of life potentially leading to an early lipid divide.Interface focus · 2019Article
- An archaeal symbiont-host association from the deep terrestrial subsurface.The ISME journal · 2019Article
- Distinct Physiological Roles of the Three Ferredoxins Encoded in the Hyperthermophilic ArchaeonmBio · 2019Article
- ConvertingProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Discovery of novel geranylgeranyl reductases and characterization of their substrate promiscuity.Biotechnology for biofuels · 2018Article
- Lipid sugar carriers at the extremes: The phosphodolichols Archaea use in N-glycosylation.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Genome-wide gene expression and RNA half-life measurements allow predictions of regulation and metabolic behavior in Methanosarcina acetivorans.BMC genomics · 2016Article
- Biosynthesis of archaeal membrane ether lipids.Frontiers in microbiology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Archaea produce membrane lipids that typically possess fully saturated isoprenoid hydrocarbon chains attached to the glycerol moiety via ether bonds. They are functionally similar to, but structurally and biosynthetically distinct from, the fatty acid-based membrane lipids of bacteria and eukaryotes. It is believed that the characteristic lipid structure helps archaea survive under severe conditions such as extremely low or high pH, high salt concentrations, and/or high temperatures. We detail here the first successful production of an intact archaeal membrane lipid, which has fully saturated isoprenoid chains, in bacterial cells. The introduction of six phospholipid biosynthetic genes from a methanogenic archaeon, Methanosarcina acetivorans, in Escherichia coli enabled the host bacterium to synthesize the archaeal lipid, i.e., diphytanylglyceryl phosphoglycerol, while a glycerol modification of the phosphate group was probably catalyzed by endogenous E. coli enzymes. Reduction of the isoprenoid chains occurred only when archaeal ferredoxin was expressed with geranylgeranyl reductase, suggesting the role of ferredoxin as a specific electron donor for the reductase. This report is the first identification of a physiological reducer for archaeal geranylgeranyl reductase. On the other hand, geranylgeranyl reductase from the thermoacidophilic archaeon Sulfolobus acidocaldarius could, by itself, replace both its orthologue and ferredoxin from M. acetivorans, which indicated that an endogenous redox system of E. coli reduced the enzyme.
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