ArticlePlant physiology2014
Deletion mutagenesis identifies a haploinsufficient role for γ-zein in opaque2 endosperm modification.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 50 citations in OpenAlex.
- Identification of QTLs associated withFrontiers in plant science · 2025Article
- A Tomato EMS-Mutagenized Population Provides New Valuable Resources for Gene Discovery and Breeding of Developmental Traits.Plants (Basel, Switzerland) · 2022Article
- StresSeed: The Unfolded Protein Response During Seed Development.Frontiers in plant science · 2022Review
- Maize Endosperm Development: Tissues, Cells, Molecular Regulation and Grain Quality Improvement.Frontiers in plant science · 2022Review
- A Proteomic Approach to Identify Zein Proteins upon Eco-Friendly Ultrasound-Based Extraction.Biomolecules · 2021Article
- Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize.Scientific reports · 2021Article
- ThePlant physiology · 2020Article
- Comparison and Characterization of Mutations Induced by Gamma-Ray and Carbon-Ion Irradiation in Rice (G3 (Bethesda, Md.) · 2019Article
- NAC-type transcription factors regulate accumulation of starch and protein in maize seeds.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A Megabase-Scale Deletion is Associated with Phenotypic Variation of Multiple Traits in Maize.Genetics · 2019Article
- High frequency DNA rearrangement atCommunications biology · 2019Article
- An Exome-seq Based Tool for Mapping and Selection of Candidate Genes in Maize Deletion Mutants.Genomics, proteomics & bioinformatics · 2018Article
- Maize opaque mutants are no longer so opaque.Plant reproduction · 2018Review
- Induction and recovery of copy number variation in banana through gamma irradiation and low-coverage whole-genome sequencing.Plant biotechnology journal · 2018Article
- Genetic Variability Induced by Gamma Rays and Preliminary Results of Low-Cost TILLING on MFrontiers in plant science · 2018Article
- Article
- Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.Journal of experimental botany · 2016Article
- A Population of Deletion Mutants and an Integrated Mapping and Exome-seq Pipeline for Gene Discovery in Maize.G3 (Bethesda, Md.) · 2016Article
- Gene duplication confers enhanced expression of 27-kDa γ-zein for endosperm modification in quality protein maize.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Introgression of opaque2 into Waxy Maize Causes Extensive Biochemical and Proteomic Changes in Endosperm.PloS one · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quality Protein Maize (QPM) is a hard kernel variant of the high-lysine mutant opaque2. Using γ-irradiation, we created opaque QPM variants to identify opaque2 modifier genes and to investigate deletion mutagenesis combined with Illumina sequencing as a maize (Zea mays) functional genomics tool. A K0326Y QPM deletion mutant was null for the 27- and 50-kD γ-zeins and abolished vitreous endosperm formation. Illumina exon and RNA sequencing revealed a 1.2-megabase pair deletion encompassing the 27- and 50-kD γ-zein genes on chromosome 7 and a deletion of at least 232 kb on chromosome 9. Protein body number was reduced by over 90%, while protein body size is similar to the wild type. Kernels hemizygous for the γ-zein deletion had intermediate 27- and 50-kD γ-zein levels and were semivitreous, indicating haploinsufficiency of these gene products in opaque2 endosperm modification. The γ-zein deletion further increased lysine in QPM in its homozygous and hemizygous states. This work identifies 27-kD γ-zein as an opaque2 modifier gene within the largest QPM quantitative trait locus and may suggest the 50-kD γ-zein also contributes to this quantitative trait locus. It further demonstrates that genome-wide deletions in nonreference maize lines can be identified through a combination of assembly of Illumina reads against the B73 genome and integration of RNA sequencing data.
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