ArticlePlant physiology2014
RBF1, a plant homolog of the bacterial ribosome-binding factor RbfA, acts in processing of the chloroplast 16S ribosomal RNA.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025Review
- Defective Processing of Cytoplasmic and Chloroplast Ribosomal RNA in the Absence of Arabidopsis DXO1.Plant, cell & environment · 2025Article
- White and green striate leaves 1, predicted to encode a 16S rRNA processing protein, plays a critical role in the processing of chloroplast ribosomes in maize (Molecular breeding : new strategies in plant improvement · 2023Article
- Plant organellar RNA maturation.The Plant cell · 2023Review
- Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA fromInternational journal of molecular sciences · 2023Article
- Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria.Microorganisms · 2022Review
- The versatile interactome of chloroplast ribosomes revealed by affinity purification mass spectrometry.Nucleic acids research · 2021Article
- Mutation of theFrontiers in plant science · 2021Article
- The Arabidopsis AAC Proteins CIL and CIA2 Are Sub-functionalized Paralogs Involved in Chloroplast Development.Frontiers in plant science · 2021Article
- The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development.Nucleic acids research · 2020Article
- The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis inPlants (Basel, Switzerland) · 2020Article
- The Arabidopsis Protein CGL20 Is Required for Plastid 50S Ribosome Biogenesis.Plant physiology · 2020Article
- Article
- Disentangling the sites of non-photochemical quenching in vascular plants.Nature plants · 2019Article
- Coexpression network revealing the plasticity and robustness of population transcriptome during the initial stage of domesticating energy crop Miscanthus lutarioriparius.Plant molecular biology · 2018Article
- CHLOROPLAST RIBOSOME ASSOCIATED Supports Translation under Stress and Interacts with the Ribosomal 30S Subunit.Plant physiology · 2018Article
- Plastid translation is essential for lateral root stem cell patterning inBiology open · 2018Article
- The DEAD-box RNA Helicase RH50 Is a 23S-4.5S rRNA Maturation Factor that Functionally Overlaps with the Plastid Signaling Factor GUN1.Plant physiology · 2018Article
- Shine-Dalgarno Sequences Play an Essential Role in the Translation of Plastid mRNAs in Tobacco.The Plant cell · 2017Article
- Lethal albinic seedling, encoding a threonyl-tRNA synthetase, is involved in development of plastid protein synthesis system in rice.Plant cell reports · 2017Article
Corrections and comments
- Erratum issuedCORRECTIONS.2015
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Plastids (chloroplasts) possess 70S ribosomes that are very similar in structure and function to the ribosomes of their bacterial ancestors. While most components of the bacterial ribosome (ribosomal RNAs [rRNAs] and ribosomal proteins) are well conserved in the plastid ribosome, little is known about the factors mediating the biogenesis of plastid ribosomes. Here, we have investigated a putative homolog of the bacterial RbfA (for ribosome-binding factor A) protein that was identified as a cold-shock protein and an auxiliary factor acting in the 5' maturation of the 16S rRNA. The unicellular green alga Chlamydomonas reinhardtii and the vascular plant Arabidopsis (Arabidopsis thaliana) both encode a single RbfA-like protein in their nuclear genomes. By generating specific antibodies against this protein, we show that the plant RbfA-like protein functions exclusively in the plastid, where it is associated with thylakoid membranes. Analysis of mutants for the corresponding gene (termed RBF1) reveals that the gene function is essential for photoautotrophic growth. Weak mutant alleles display reduced levels of plastid ribosomes, a specific depletion in 30S ribosomal subunits, and reduced activity of plastid protein biosynthesis. Our data suggest that, while the function in ribosome maturation and 16S rRNA 5' end processing is conserved, the RBF1 protein has assumed an additional role in 3' end processing. Together with the apparent absence of a homologous protein from plant mitochondria, our findings illustrate that the assembly process of the 70S ribosome is not strictly conserved and has undergone some modifications during organelle evolution.
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