ArticleTransgenic research2014
Inducible, tightly regulated and non-leaky neuronal gene expression in mice.
Article in Transgenic research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
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- PLP1-Targeting Antisense Oligonucleotides Improve FOXG1 Syndrome Mice.International journal of molecular sciences · 2024Article
- Tetracycline response element driven Cre causes ectopic recombinase activity independent of transactivator element.Molecular metabolism · 2022Article
- Article
- Photoactivatable Cre knock-in mice for spatiotemporal control of genetic engineering in vivo.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection.Journal of visualized experiments : JoVE · 2018Article
- Overexpression of miR-210 and its significance in ischemic tissue damage.Scientific reports · 2017Article
- Synthetic circuits that process multiple light and chemical signal inputs.BMC systems biology · 2017Article
- Structure-function integrity of the adult hippocampus depends on the transcription factor Bcl11b/Ctip2.Genes, brain, and behavior · 2016Article
- Expression of A152T human tau causes age-dependent neuronal dysfunction and loss in transgenic mice.EMBO reports · 2016Article
- A Tet-on and Cre-loxP Based Genetic Engineering System for Convenient Recycling of Selection Markers in Penicillium oxalicum.Frontiers in microbiology · 2016Article
- FTD and ALS--translating mouse studies into clinical trials.Nature reviews. Neurology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Tetracycline (Tet)-controlled inducible system is the most widely used reversible system for transgene expression in mice with over 500 lines created to date. Although this system has been optimized over the years, it still has limitations such as residual transgene expression when turned off, referred to as leakiness. Here, we present a series of new Tet-OFF transgenic mice based on the second generation tetracycline-responsive transactivator system. The tTA-Advanced (tTA2(S)) is expressed under control of the neuron-specific Thy1.2 promoter (Thy-OFF), to regulate expression in the mouse brain. In addition, we generated a lacZ reporter line, utilizing the P tight Tet-responsive promoter (P(tight)-lacZ), to test our system. Two Thy-OFF transgenic lines displaying two distinct patterns of expression were selected. Oral doxycycline treatment of Thy-OFF/P tight-lacZ mice demonstrated tight transgene regulation with no leak expression. These new Thy-OFF mice are valuable for studies in a broad range of neurodegenerative diseases such as Alzheimer's disease and related forms of dementia, where control of transgene expression is critical to understanding mechanisms underlying the disease. Furthermore, P tight-lacZ reporter mice may be widely applicable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.