ArticleGenetics2014
Estimation of the spontaneous mutation rate per nucleotide site in a Drosophila melanogaster full-sib family.
Article in Genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 162 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
162 citing papers in PubMed, 333 citations in OpenAlex.
- Intersecting experimental evolution and CRISPR screens to identify novel toxin resistance loci.eLife · 2026Article
- Differential Demographic Trajectories and Polygenic Adaptation to Monsoon Seasonality in Sky Island Hercules Beetle Populations of the American Southwest.Molecular ecology · 2026Article
- Adaptive introgression between two Drosophila species enhances heat tolerance despite barriers to gene flow.PLoS biology · 2026Article
- Gene-level complexity explains genome-wide variation in the distribution of fitness effects.Molecular biology and evolution · 2026Article
- Estimating the evolutionary fitness of specific synonymous codon changes.Molecular biology and evolution · 2026Article
- The Effect of Age and Sex on the Rate of Germline Mutations in Barn Owls.Genome biology and evolution · 2026Article
- Cessation of Gene Flow Associated With the Reduction of a Sexually Selected Phenotype in the Island Stag Beetle.Molecular ecology · 2026Article
- What sets the mutation rate of a cell type in an animal species?PLoS biology · 2026Article
- Leveraging large family analyses for more accurate de novo mutation detection.Biologia futura · 2026Article
- Examining the Role of a Chromosomal Inversion in Accumulating Adaptive and Barrier Loci in a Cold-adapted Insect Species.Genome biology and evolution · 2026Article
- Cryptic Diversity and Impacts of Domestication in the Black Soldier Fly (Hermetia illucens) Genome.Genome biology and evolution · 2026Article
- Genetic Footprints of Seasonal Fluctuating Selection: A Comparison With Established Selection Forms.Genome biology and evolution · 2026Article
- Latitudinal Clines in Gene Flow and Demographic Stability Reveal Drivers of Microendemism in a Radiation of Alpine Grasshoppers.Molecular ecology · 2026Article
- ThebioRxiv : the preprint server for biology · 2026Article
- The distribution of fitness effects of new mutations in regulatory regions of thebioRxiv : the preprint server for biology · 2026Article
- Transposable elements contribute substantially to naturally occurring genetic lethality in Drosophila melanogaster.PLoS biology · 2026Article
- Early hominin arrival in Southeast Asia triggered the evolution of major human malaria vectors.Scientific reports · 2026Article
- Article
- The mutation landscape of Daphnia obtusa reveals evolutionary forces shaping genome stability.Molecular biology and evolution · 2026Article
- Glacial Legacies-How Refugial Dynamics Shaped the Evolution of the Alpine Endemic Bush-Cricket Anonconotus italoaustriacus.Molecular ecology · 2026Article
102 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We employed deep genome sequencing of two parents and 12 of their offspring to estimate the mutation rate per site per generation in a full-sib family of Drosophila melanogaster recently sampled from a natural population. Sites that were homozygous for the same allele in the parents and heterozygous in one or more offspring were categorized as candidate mutations and subjected to detailed analysis. In 1.23 × 10(9) callable sites from 12 individuals, we confirmed six single nucleotide mutations. We estimated the false negative rate in the experiment by generating synthetic mutations using the empirical distributions of numbers of nonreference bases at heterozygous sites in the offspring. The proportion of synthetic mutations at callable sites that we failed to detect was <1%, implying that the false negative rate was extremely low. Our estimate of the point mutation rate is 2.8 × 10(-9) (95% confidence interval = 1.0 × 10(-9) - 6.1 × 10(-9)) per site per generation, which is at the low end of the range of previous estimates, and suggests an effective population size for the species of ∼1.4 × 10(6). At one site, point mutations were present in two individuals, indicating that there had been a premeiotic mutation cluster, although surprisingly one individual had a G→A transition and the other a G→T transversion, possibly associated with error-prone mismatch repair. We also detected three short deletion mutations and no insertions, giving a deletion mutation rate of 1.2 × 10(-9) (95% confidence interval = 0.7 × 10(-9) - 11 × 10(-9)).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.