ArticleNature biotechnology2013
Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells.
Article in Nature biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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Who cites it
111 citing papers in PubMed.
- A medium throughput approach for single cell copy number variation sequencing towards efficient application in clinics.Journal of advanced research · 2026Article
- Enrichment of horizontally transferred gene clusters in bacterial extracellular vesicles via non lytic mechanisms.The ISME journal · 2025Article
- Single-cell somatic copy number variants in brain using different amplification methods and reference genomes.Communications biology · 2024Article
- Deciphering breast cancer dynamics: insights from single-cell and spatial profiling in the multi-omics era.Biomarker research · 2024Review
- Fabrication and Cell Culture Applications of Core-Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations.Advanced healthcare materials · 2023Article
- Single-cell somatic copy number variants in brain using different amplification methods and reference genomes.bioRxiv : the preprint server for biology · 2023Article
- Chromosome instability and aneuploidy in the mammalian brain.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Review
- Barcoded multiple displacement amplification for high coverage sequencing in spatial genomics.Nature communications · 2023Article
- Technological and computational approaches to detect somatic mosaicism in epilepsy.Neurobiology of disease · 2023Review
- Back to Basics: A Simplified Improvement to Multiple Displacement Amplification for Microbial Single-Cell Genomics.International journal of molecular sciences · 2023Article
- Research progress of single-cell sequencing in tuberculosis.Frontiers in immunology · 2023Review
- Chimera: The spoiler in multiple displacement amplification.Computational and structural biotechnology journal · 2023Review
- Somatic CNV Detection by Single-Cell Whole-Genome Sequencing in Postmortem Human Brain.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Droplet Microfluidics Enables Tracing of Target Cells at the Single-Cell Transcriptome Resolution.Bioengineering (Basel, Switzerland) · 2022Article
- Agarose gel microcapsules enable easy-to-prepare, picolitre-scale, single-cell genomics, yielding high-coverage genome sequences.Scientific reports · 2022Article
- Validation of the application of gel beads-based single-cell genome sequencing platform to soil and seawater.ISME communications · 2022Article
- Single-cell genomics reveals population structures fromMicrobial genomics · 2022Article
- Review
- Single-Nucleus RNA-seq of Normal-Appearing Brain Regions in Relapsing-Remitting vs. Secondary Progressive Multiple Sclerosis: Implications for the Efficacy of Fingolimod.Frontiers in cellular neuroscience · 2022Article
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
51 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
Abstract
Genome sequencing of single cells has a variety of applications, including characterizing difficult-to-culture microorganisms and identifying somatic mutations in single cells from mammalian tissues. A major hurdle in this process is the bias in amplifying the genetic material from a single cell, a procedure known as polymerase cloning. Here we describe the microwell displacement amplification system (MIDAS), a massively parallel polymerase cloning method in which single cells are randomly distributed into hundreds to thousands of nanoliter wells and their genetic material is simultaneously amplified for shotgun sequencing. MIDAS reduces amplification bias because polymerase cloning occurs in physically separated, nanoliter-scale reactors, facilitating the de novo assembly of near-complete microbial genomes from single Escherichia coli cells. In addition, MIDAS allowed us to detect single-copy number changes in primary human adult neurons at 1- to 2-Mb resolution. MIDAS can potentially further the characterization of genomic diversity in many heterogeneous cell populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.