ArticleApplied and environmental microbiology2014
Metaproteomics analysis reveals the adaptation process for the chicken gut microbiota.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 77 citations in OpenAlex.
- Threats and Opportunities When Using Chickens as a Model for Host-Microbiota Studies.Microorganisms · 2026Review
- Exploring the Mechanism ofBiology · 2025Article
- A Two Bacteriocinogenic Ligilactobacillus Strain Association Inhibits Growth, Adhesion, and Invasion of Salmonella in a Simulated Chicken Gut Environment.Probiotics and antimicrobial proteins · 2024Article
- Large-Scale Field Trials of anVaccines · 2024Article
- The Functional Characteristics of Goat Cheese Microbiota from a One-Health Perspective.International journal of molecular sciences · 2022Article
- Impact of the gastrointestinal microbiome and fermentation metabolites on broiler performance.Poultry science · 2022Review
- Breed differences in the expression levels of gga-miR-222a in laying hens influenced HMicrobiome · 2021Article
- A slurry microcosm study on the interaction between antibiotics and soil bacterial community.Heliyon · 2020Article
- Article
- Effect of Lactobacillus acidophilus D2/CSL (CECT 4529) supplementation in drinking water on chicken crop and caeca microbiome.PloS one · 2020Article
- Triflic Acid Treatment Enables LC-MS/MS Analysis of Insoluble Bacterial Biomass.Journal of proteome research · 2018Article
- Antibiotic-mediated changes in the fecal microbiome of broiler chickens define the incidence of antibiotic resistance genes.Microbiome · 2018Article
- Article
- Current Perspectives of the Chicken Gastrointestinal Tract and Its Microbiome.Computational and structural biotechnology journal · 2018Review
- Chicken Gut Microbiota: Importance and Detection Technology.Frontiers in veterinary science · 2018Review
- Dietary changes in nutritional studies shape the structural and functional composition of the pigs' fecal microbiome-from days to weeks.Microbiome · 2017Article
- Article
- Microbial Shifts in the Intestinal Microbiota ofFrontiers in microbiology · 2017Article
- Identification of temperature regulated factors ofAIMS microbiology · 2017Article
- Characterising the interspecific variations and convergence of gut microbiota in Anseriformes herbivores at wintering areas.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The animal gastrointestinal tract houses a large microbial community, the gut microbiota, that confers many benefits to its host, such as protection from pathogens and provision of essential metabolites. Metagenomic approaches have defined the chicken fecal microbiota in other studies, but here, we wished to assess the correlation between the metagenome and the bacterial proteome in order to better understand the healthy chicken gut microbiota. Here, we performed high-throughput sequencing of 16S rRNA gene amplicons and metaproteomics analysis of fecal samples to determine microbial gut composition and protein expression. 16 rRNA gene sequencing analysis identified Clostridiales, Bacteroidaceae, and Lactobacillaceae species as the most abundant species in the gut. For metaproteomics analysis, peptides were generated by using the Fasp method and subsequently fractionated by strong anion exchanges. Metaproteomics analysis identified 3,673 proteins. Among the most frequently identified proteins, 380 proteins belonged to Lactobacillus spp., 155 belonged to Clostridium spp., and 66 belonged to Streptococcus spp. The most frequently identified proteins were heat shock chaperones, including 349 GroEL proteins, from many bacterial species, whereas the most abundant enzymes were pyruvate kinases, as judged by the number of peptides identified per protein (spectral counting). Gene ontology and KEGG pathway analyses revealed the functions and locations of the identified proteins. The findings of both metaproteomics and 16S rRNA sequencing analyses are discussed.
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