ArticleApplied and environmental microbiology2014
Harnessing genetic diversity in Saccharomyces cerevisiae for fermentation of xylose in hydrolysates of alkaline hydrogen peroxide-pretreated biomass.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Biotechnological applications of D-xylose-fermenting autochthonous yeasts in lignocellulosic biorefineries.Biotechnology for biofuels and bioproducts · 2026Review
- Developmentally specific physiological and metabolic responses support drought resilience in switchgrass and constrain biofuel yield.Journal of experimental botany · 2026Article
- Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces.Biotechnology for biofuels and bioproducts · 2024Article
- Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.Bioprocess and biosystems engineering · 2023Article
- Review
- Review
- Natural Variation in the Multidrug Efflux PumpGenetics · 2018Article
- Augmenting Pentose Utilization and Ethanol Production of NativeFrontiers in bioengineering and biotechnology · 2018Article
- Release of cell wall phenolic esters during hydrothermal pretreatment of rice husk and rice straw.Biotechnology for biofuels · 2018Article
- Industrial Relevance of Chromosomal Copy Number Variation in Saccharomyces Yeasts.Applied and environmental microbiology · 2017Review
- Genome sequence and physiological analysis of Yamadazyma laniorum f.a. sp. nov. and a reevaluation of the apocryphal xylose fermentation of its sister species, Candida tenuis.FEMS yeast research · 2017Article
- Hybridization and adaptive evolution of diverseBiotechnology for biofuels · 2017Article
- Genome Sequence and Analysis of a Stress-Tolerant, Wild-Derived Strain of Saccharomyces cerevisiae Used in Biofuels Research.G3 (Bethesda, Md.) · 2016Article
- Mechanism of imidazolium ionic liquids toxicity in Saccharomyces cerevisiae and rational engineering of a tolerant, xylose-fermenting strain.Microbial cell factories · 2016Article
- Evolved hexose transporter enhances xylose uptake and glucose/xylose co-utilization in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris.Environmental science & technology · 2015Article
- Comparative genomics of Saccharomyces cerevisiae natural isolates for bioenergy production.Genome biology and evolution · 2014Article
- Coupling alkaline pre-extraction with alkaline-oxidative post-treatment of corn stover to enhance enzymatic hydrolysis and fermentability.Biotechnology for biofuels · 2014Article
- Article
- Studying the rapid bioconversion of lignocellulosic sugars into ethanol using high cell density fermentations with cell recycle.Biotechnology for biofuels · 2014Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fermentation of lignocellulose-derived sugars, particularly xylose, into ethanol by the yeast Saccharomyces cerevisiae is known to be inhibited by compounds produced during feedstock pretreatment. We devised a strategy that combined chemical profiling of pretreated feedstocks, high-throughput phenotyping of genetically diverse S. cerevisiae strains isolated from a range of ecological niches, and directed engineering and evolution against identified inhibitors to produce strains with improved fermentation properties. We identified and quantified for the first time the major inhibitory compounds in alkaline hydrogen peroxide (AHP)-pretreated lignocellulosic hydrolysates, including Na(+), acetate, and p-coumaric (pCA) and ferulic (FA) acids. By phenotyping these yeast strains for their abilities to grow in the presence of these AHP inhibitors, one heterozygous diploid strain tolerant to all four inhibitors was selected, engineered for xylose metabolism, and then allowed to evolve on xylose with increasing amounts of pCA and FA. After only 149 generations, one evolved isolate, GLBRCY87, exhibited faster xylose uptake rates in both laboratory media and AHP switchgrass hydrolysate than its ancestral GLBRCY73 strain and completely converted 115 g/liter of total sugars in undetoxified AHP hydrolysate into more than 40 g/liter ethanol. Strikingly, genome sequencing revealed that during the evolution from GLBRCY73, the GLBRCY87 strain acquired the conversion of heterozygous to homozygous alleles in chromosome VII and amplification of chromosome XIV. Our approach highlights that simultaneous selection on xylose and pCA or FA with a wild S. cerevisiae strain containing inherent tolerance to AHP pretreatment inhibitors has potential for rapid evolution of robust properties in lignocellulosic biofuel production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.