ArticleScientific reports2013
Live single cell functional phenotyping in droplet nano-liter reactors.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- High-throughput combinatorial screening of antiplatelet drugs for personalized medicine.Microsystems & nanoengineering · 2026Article
- Imaging-Based Efficacy Evaluation of Cancer Immunotherapy in Engineered Tumor Platforms and Tumor Organoids.Advanced healthcare materials · 2024Review
- An innovative single-cell approach for phenotyping and functional genotyping of CAR NK cells.Journal for immunotherapy of cancer · 2024Article
- High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays.Nature biomedical engineering · 2023Article
- Attomole-Level Multiplexed Detection of Neurochemicals in Picoliter Droplets by On-Chip Nanoelectrospray Ionization Coupled to Mass Spectrometry.Analytical chemistry · 2022Article
- Hydrogels for Single-Cell Microgel Production: Recent Advances and Applications.Frontiers in bioengineering and biotechnology · 2022Review
- The future of antibiotics begins with discovering new combinations.Annals of the New York Academy of Sciences · 2021Review
- Efficient Generation of Microdroplets Using Tail Breakup Induced with Multi-Branch Channels.Molecules (Basel, Switzerland) · 2021Article
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Engineered Tools to Study Intercellular Communication.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Quantifying the efficacy of checkpoint inhibitors on CD8Cell death & disease · 2020Article
- Single platelet variability governs population sensitivity and initiates intrinsic heterotypic responses.Communications biology · 2020Article
- How single-cell immunology is benefiting from microfluidic technologies.Microsystems & nanoengineering · 2020Review
- Functional TCR T cell screening using single-cell droplet microfluidics.Lab on a chip · 2018Article
- Combinatorial drug discovery in nanoliter droplets.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Simple, low-cost fabrication of acrylic based droplet microfluidics and its use to generate DNA-coated particles.Scientific reports · 2018Article
- Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution.Scientific reports · 2018Article
- Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment.Chemical reviews · 2017Review
- Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
While single cell heterogeneity is present in all biological systems, most studies cannot address it due to technical limitations. Here we describe a nano-liter droplet microfluidic-based approach for stimulation and monitoring of surface and secreted markers of live single immune dendritic cells (DCs) as well as monitoring the live T cell/DC interaction. This nano-liter in vivo simulating microenvironment allows delivering various stimuli reagents to each cell and appropriate gas exchanges which are necessary to ensure functionality and viability of encapsulated cells. Labeling bioassay and microsphere sensors were integrated into nano-liter reaction volume of the droplet to monitor live single cell surface markers and secretion analysis in the time-dependent fashion. Thus live cell stimulation, secretion and surface monitoring can be obtained simultaneously in distinct microenvironment, which previously was possible using complicated and multi-step in vitro and in vivo live-cell microscopy, together with immunological studies of the outcome secretion of cellular function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.