ArticleJournal of lipid research2014
ABCG1-mediated generation of extracellular cholesterol microdomains.
Article in Journal of lipid research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Modern Concepts of the Role of Fine Particles (PM 2.5) in the Genesis of Atherosclerosis and Myocardial Damage: Clinical and Epidemiological Data, the Main Pathophysiological Mechanisms.Current cardiology reviews · 2023Review
- Snapshots of ABCG1 and ABCG5/G8: A Sterol's Journey to Cross the Cellular Membranes.International journal of molecular sciences · 2022Review
- The Role of Fine Particles (PM 2.5) in the Genesis of Atherosclerosis and Myocardial Damage: Emphasis on Clinical and Epidemiological Data, and Pathophysiological Mechanisms.Cardiology research · 2022Review
- Global research trends in atherosclerosis: A bibliometric and visualized study.Frontiers in cardiovascular medicine · 2022Article
- U-Shaped Association Between Serum Uric Acid Level and Hypertensive Heart Failure: A Genetic Matching Case-Control Study.Frontiers in cardiovascular medicine · 2021Article
- Probucol treatment is associated with an ABCA1-independent mechanism of cholesterol efflux to lipid poor apolipoproteins from foam cell macrophages.BBA advances · 2021Article
- Cultured macrophages transfer surplus cholesterol into adjacent cells in the absence of serum or high-density lipoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- LCAT, ApoD, and ApoA1 Expression and Review of Cholesterol Deposition in the Cornea.Biomolecules · 2019Article
- Article
- Ultramorphological analysis of plaque advancement and cholesterol crystal formation in Ldlr knockout mouse atherosclerosis.Atherosclerosis · 2019Article
- Low Phytanic Acid-Concentrated DHA Prevents Cognitive Deficit and Regulates Alzheimer Disease Mediators in an ApoENutrients · 2018Article
- Macrophages release plasma membrane-derived particles rich in accessible cholesterol.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Two polymorphic cholesterol monohydrate crystal structures form in macrophage culture models of atherosclerosis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Macrophages Shed Excess Cholesterol in Unique Extracellular Structures Containing Cholesterol Microdomains.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- ABCA1 (ATP-Binding Cassette Transporter A1) Mediates ApoA-I (Apolipoprotein A-I) and ApoA-I Mimetic Peptide Mobilization of Extracellular Cholesterol Microdomains Deposited by Macrophages.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages.Journal of visualized experiments : JoVE · 2016Article
- ABCA1 contributes to macrophage deposition of extracellular cholesterol.Journal of lipid research · 2015Article
- Advances in the Study of the Antiatherogenic Function and Novel Therapies for HDL.International journal of molecular sciences · 2015Review
- Macrophages, extracellular matrix, and lipoproteins in arterial cholesterol balance.Journal of lipid research · 2014Article
- Adaptive responses induced by 24S-hydroxycholesterol through liver X receptor pathway reduce 7-ketocholesterol-caused neuronal cell death.Redox biology · 2013Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Previous studies have demonstrated that the ATP-binding cassette transporters (ABC)A1 and ABCG1 function in many aspects of cholesterol efflux from macrophages. In this current study, we continued our investigation of extracellular cholesterol microdomains that form during enrichment of macrophages with cholesterol. Human monocyte-derived macrophages and mouse bone marrow-derived macrophages, differentiated with macrophage colony-stimulating factor (M-CSF) or granulocyte macrophage colony-stimulation factor (GM-CSF), were incubated with acetylated LDL (AcLDL) to allow for cholesterol enrichment and processing. We utilized an anti-cholesterol microdomain monoclonal antibody to reveal pools of unesterified cholesterol, which were found both in the extracellular matrix and associated with the cell surface, that we show function in reverse cholesterol transport. Coincubation of AcLDL with 50 μg/ml apoA-I eliminated all extracellular and cell surface-associated cholesterol microdomains, while coincubation with the same concentration of HDL only removed extracellular matrix-associated cholesterol microdomains. Only at an HDL concentration of 200 µg/ml did HDL eliminate the cholesterol microdomains that were cell-surface associated. The deposition of cholesterol microdomains was inhibited by probucol, but it was increased by the liver X receptor (LXR) agonist TO901317, which upregulates ABCA1 and ABCG1. Extracellular cholesterol microdomains did not develop when ABCG1-deficient mouse bone marrow-derived macrophages were enriched with cholesterol. Our findings show that generation of extracellular cholesterol microdomains is mediated by ABCG1 and that reverse cholesterol transport occurs not only at the cell surface but also within the extracellular space.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.