ArticleJournal of structural biology2014
Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography.
Article in Journal of structural biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Microscopy in mitochondrial research - a comprehensive review.Journal of cell science · 2026Review
- Uncovering synaptic and cellular nanoarchitecture of brain tissue via seamlessbioRxiv : the preprint server for biology · 2025Article
- Integrated multimodality microscope for accurate and efficient target-guided cryo-lamellae preparation.Nature methods · 2023Article
- ArtiaX: An electron tomography toolbox for the interactive handling of sub-tomograms in UCSF ChimeraX.Protein science : a publication of the Protein Society · 2022Article
- Bridging length scales from molecules to the whole organism by cryoCLEM and cryoET.Faraday discussions · 2022Article
- Waffle Method: A general and flexible approach for improving throughput in FIB-milling.Nature communications · 2022Article
- Electron microscopy for imaging organelles in plants and algae.Plant physiology · 2022Article
- An Overview of Microcrystal Electron Diffraction (MicroED).Annual review of biochemistry · 2021Review
- CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy.Journal of structural biology · 2021Article
- Cryo-FIB preparation of whole cells and tissue for cryo-TEM: use of high-pressure frozen specimens in tubes and planchets.Journal of microscopy · 2021Article
- An introduction to cryo-FIB-SEM cross-sectioning of frozen, hydrated Life Science samples.Journal of microscopy · 2021Article
- Structure of the Bacterial Cellulose Ribbon and Its Assembly-Guiding Cytoskeleton by Electron Cryotomography.Journal of bacteriology · 2021Article
- Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm.Methods in cell biology · 2021Article
- Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography.Nature protocols · 2020Article
- A cryo-FIB lift-out technique enables molecular-resolution cryo-ET within native Caenorhabditis elegans tissue.Nature methods · 2019Article
- Article
- Review
- Fine details in complex environments: the power of cryo-electron tomography.Biochemical Society transactions · 2018Review
- Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM.Journal of structural biology · 2017Article
- Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Vitreous freezing offers a way to study cells and tissue in a near-native state by cryo-transmission electron microscopy (cryo-TEM), which is important when structural information at the macromolecular level is required. Many cells - especially those in tissue - are too thick to study intact in the cryo-TEM. Cryo focused-ion-beam (cryo-FIB) milling is being used in a few laboratories to thin vitreously frozen specimens, thus avoiding the artifacts and difficulties of cryo-ultramicrotomy. However, the technique is challenging because of the need to avoid devitrification and frost accumulation during the entire process, from the initial step of freezing to the final step of loading the specimen into the cryo-TEM. We present a robust workflow that makes use of custom fixtures and devices that can be used for high-pressure-frozen bulk tissue samples as well as for samples frozen on TEM grids.
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Registered trials
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