Evidence map›Paper›PMID 24211762›Full record

ArticleDevelopmental biology2014

Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.

L J Dawes, Y Sugiyama, F J Lovicu, C G Harris, E J Shelley, J W McAvoy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Secreted Frizzled-related proteins in animal development.Histochemistry and cell biology · 2025
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Lens regenerationiScience · 2023
    Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Review
  12. Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019
    Review
  13. Article
  14. Article
  15. Review
  16. Review
  17. The lens growth process.Progress in retinal and eye research · 2017
    Review
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

L J DawesSave Sight Institute, University of Sydney, Australia.
Y SugiyamaSave Sight Institute, University of Sydney, Australia.
F J LovicuDiscipline of Anatomy and Histology, Bosch Institute, University of Sydney, Australia.
C G HarrisSave Sight Institute, University of Sydney, Australia.
E J ShelleySave Sight Institute, University of Sydney, Australia.
J W McAvoySave Sight Institute, University of Sydney, Australia. Electronic address: john.mcavoy@sydney.edu.au.

Funding

STUDIES ON LENS DIFFERENTIATION: ROLE OF FGFR01EY003177 · NEI · UNIVERSITY OF SYDNEY · PI LOVICU, FRANK JAMES, MCAVOY, JOHNSTON W · 1985 to 2015
$1.8M
NEI NIH HHS R01 EY003177NEI NIH HHS R01EY0-3177
6 · The paper itself

Abstract

How tissues and organs develop and maintain their characteristic three-dimensional cellular architecture is often a poorly understood part of their developmental program; yet, as is clearly the case for the eye lens, precise regulation of these features can be critical for function. During lens morphogenesis cells become organized into a polarized, spheroidal structure with a monolayer of epithelial cells overlying the apical tips of elongated fiber cells. Epithelial cells proliferate and progeny that shift below the lens equator differentiate into new fibers that are progressively added to the fiber mass. It is now known that FGF induces epithelial to fiber differentiation; however, it is not fully understood how these two forms of cells assemble into their characteristic polarized arrangement. Here we show that in FGF-treated epithelial explants, elongating fibers become polarized/oriented towards islands of epithelial cells and mimic their polarized arrangement in vivo. Epithelial explants secrete Wnt5 into the culture medium and we show that Wnt5 can promote directed behavior of lens cells. We also show that these explants replicate aspects of the Notch/Jagged signaling activity that has been shown to regulate proliferation of epithelial cells in vivo. Thus, our in vitro study identifies a novel mechanism, intrinsic to the two forms of lens cells, that facilitates self-assembly into the polarized arrangement characteristic of the lens in vivo. In this way the lens, with its relatively simple cellular composition, serves as a useful model to highlight the importance of such intrinsic self-assembly mechanisms in tissue developmental and regenerative processes.

Indexed as

AnimalsBlotting, WesternCalcium-Binding ProteinsCell DifferentiationCoculture TechniquesEnzyme-Linked Immunosorbent AssayEpithelial CellsFibroblast Growth FactorsIntercellular Signaling Peptides and ProteinsJagged-1 ProteinLens, CrystallineMembrane ProteinsRatsRats, WistarReceptors, NotchSerrate-Jagged ProteinsCalcium-Binding ProteinsFibroblast Growth FactorsIntercellular Signaling Peptides and ProteinsJag1 protein, ratJagged-1 ProteinMembrane ProteinsReceptors, NotchSerrate-Jagged ProteinsWnt-5a ProteinWnt5a protein, ratWnt ProteinsDevelopment and regenerationFGFJaggedLens epithelial cellsLens fiber cellsNotchPlanar cell polarityWnt

Identifiers

PMID24211762
PMCPMC3947272

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.