ArticleCurrent biology : CB2013
Circadian yin-yang regulation and its manipulation to globally reprogram gene expression.
Article in Current biology : CB, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Cyanobacterial Circadian Clock: Molecular Mechanisms and Physiological Outputs.Plants (Basel, Switzerland) · 2026Review
- RpaA Overexpression Enhances Bioluminescence Intensity and Elevates Rhythmic Extracellular Vesicle Yield inLife (Basel, Switzerland) · 2026Article
- Revitalizing the "biology" in "chronobiology".Npj biological timing and sleep · 2026Review
- Modulatory effects of cinnamomi cortex and its components epicatechin and linalool on skin circadian rhythms.Scientific reports · 2025Article
- Two KaiABC systems control circadian oscillations in one cyanobacterium.Nature communications · 2024Article
- Clocking out and letting go to unleash green biotech applications in a photosynthetic host.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
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- Article
- Culturing Synechocystis sp. Strain PCC 6803 with N2 and CO2 in a Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance Costs.Applied and environmental microbiology · 2016Article
- Shorter and sturdier bridges between traditional Chinese medicines and modern pharmacology.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2015Article
- Cyanobacterial hydrogenases and hydrogen metabolism revisited: recent progress and future prospects.International journal of molecular sciences · 2015Review
- Giving Time Purpose: The Synechococcus elongatus Clock in a Broader Network Context.Annual review of genetics · 2015Review
- Circadian Control of Global Transcription.BioMed research international · 2015Review
- Rhythms in energy storage control the ability of the cyanobacterial circadian clock to reset.Current biology : CB · 2014Article
- Dynamic localization of the cyanobacterial circadian clock proteins.Current biology : CB · 2014Article
- Metabolic compensation and circadian resilience in prokaryotic cyanobacteria.Annual review of biochemistry · 2014Review
- Estimating the yin-yang nature of Western herbs: a potential tool based on antioxidation-oxidation theory.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2014Article
- Circadian rhythms: hijacking the cyanobacterial clock.Current biology : CB · 2013Article
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Authors and funding
8 authors.
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Abstract
backgroundThe cyanobacterial circadian program exerts genome-wide control of gene expression. KaiC undergoes rhythms of phosphorylation that are regulated by interactions with KaiA and KaiB. The phosphorylation status of KaiC is thought to mediate global transcription via output factors SasA, CikA, LabA, RpaA, and RpaB. Overexpression of kaiC has been reported to globally repress gene expression.
resultsHere, we show that the positive circadian component KaiA upregulates "subjective dusk" genes and that its overexpression deactivates rhythmic gene expression without significantly affecting growth rates in constant light. We analyze the global patterns of expression that are regulated by KaiA versus KaiC and find in contrast to the previous report of KaiC repression that there is a "yin-yang" regulation of gene expression whereby kaiA overexpression activates "dusk genes" and represses "dawn genes," whereas kaiC overexpression complementarily activates dawn genes and represses dusk genes. Moreover, continuous induction of kaiA latched KaiABC-regulated gene expression to provide constitutively increased transcript levels of diverse endogenous and heterologous genes that are expressed in the predominant subjective dusk phase. In addition to analyzing KaiA regulation of endogenous gene expression, we apply these insights to the expression of heterologous proteins whose products are of potential value, namely human proinsulin, foreign luciferase, and exogenous hydrogenase.
conclusionsBoth KaiC and KaiA complementarily contribute to the regulation of circadian gene expression via yin-yang switching. Circadian patterns can be reprogrammed by overexpression of kaiA or kaiC to constitutively enhance gene expression, and this reprogramming can improve 24/7 production of heterologous proteins that are useful as pharmaceuticals or biofuels.
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