ArticleBiomaterials2014
Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Innovative applications of multidimensional engineered hydrogels in wound healing.Journal of advanced research · 2026Review
- Biofunctional Carboxymethyl Chitosan Hydrogel Incorporating Hyaluronic Acid and RGD Peptides for Accelerated Wound Repair.Gels (Basel, Switzerland) · 2025Article
- Non-viral gene therapy using RNA interference with PDGFR-α mediated epithelial-mesenchymal transformation for proliferative vitreoretinopathy.Materials today. Bio · 2023Article
- VEGF-Encoding, Gene-Activated Collagen-Based Matrices Promote Blood Vessel Formation and Improved Wound Repair.ACS applied materials & interfaces · 2023Article
- Emerging scaffold- and cellular-based strategies for brain tissue regeneration and imaging.In vitro models · 2022Review
- Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems.Journal, genetic engineering & biotechnology · 2021Review
- Design Challenges in Polymeric Scaffolds for Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches-An Overview.Pharmaceutics · 2020Review
- Potential Applications of Nanomaterials and Technology for Diabetic Wound Healing.International journal of nanomedicine · 2020Review
- Injectable, Hyaluronic Acid-Based Scaffolds with Macroporous Architecture for Gene Delivery.Cellular and molecular bioengineering · 2019Article
- Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Bioprinting of Vascularized Tissue Scaffolds: Influence of Biopolymer, Cells, Growth Factors, and Gene Delivery.Journal of healthcare engineering · 2019Review
- Hyaluronic acid hydrogel scaffolds loaded with cationic niosomes for efficient non-viral gene delivery.RSC advances · 2018Article
- It's All in the Delivery: Designing Hydrogels for Cell and Non-viral Gene Therapies.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Review
- Correlation of the cytotoxic effects of cationic lipids with their headgroups.Toxicology research · 2018Article
- Non-viral gene delivery systems for tissue repair and regeneration.Journal of translational medicine · 2018Review
- Directing three-dimensional multicellular morphogenesis by self-organization of vascular mesenchymal cells in hyaluronic acid hydrogels.Journal of biological engineering · 2017Article
- Hydrogel as a bioactive material to regulate stem cell fate.Bioactive materials · 2016Review
- Hydrogels for brain repair after stroke: an emerging treatment option.Current opinion in biotechnology · 2016Review
- Light-triggered RNA release and induction of hMSC osteogenesis via photodegradable, dual-crosslinked hydrogels.Nanomedicine (London, England) · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The lack of vascularization within tissue-engineered constructs remains the primary cause of construct failure following implantation. Porous constructs have been successful in allowing for vessel infiltration without requiring extensive matrix degradation. We hypothesized that the rate and maturity of infiltrating vessels could be enhanced by complementing the open pore structure with the added delivery of DNA encoding for angiogenic growth factors. Both 100 and 60 μm porous and non-porous hyaluronic acid hydrogels loaded with pro-angiogenic (pVEGF) or reporter (pGFPluc) plasmid nanoparticles were used to study the effects of pore size and DNA delivery on angiogenesis in a mouse subcutaneous implant model. GFP-expressing transfected cells were found inside all control hydrogels over the course of the study, although transfection levels peaked by week 3 for 100 and 60 μm porous hydrogels. Transfection in non-porous hydrogels continued to increase over time corresponding with continued surface degradation. pVEGF transfection levels were not high enough to enhance angiogenesis by increasing vessel density, maturity, or size, although by 6 weeks for all pore size hydrogels more hydrogel implants were positive for vascularization when pVEGF polyplexes were incorporated compared to control hydrogels. Pore size was found to be the dominant factor in determining the angiogenic response with 60 μm porous hydrogels having more vessels/area present than 100 μm porous hydrogels at the initial onset of angiogenesis at 3 weeks. The results of this study show promise for the use of polyplex loaded porous hydrogels to transfect infiltrating cells in vivo and guide tissue regeneration and repair.
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Registered trials
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