ArticleBiophysical journal2013
Gloeobacter rhodopsin, limitation of proton pumping at high electrochemical load.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Rhobot-Screen: an integrated robotic platform for functional screening of rhodopsin variants.BMC biology · 2026Article
- Light-harvesting by antenna-containing xanthorhodopsin from an Antarctic Pseudanabaenaceae cyanobacterium.Communications biology · 2025Article
- Driving forces of proton-pumping rhodopsins.Biophysical journal · 2024Article
- Improved production of β-carotene in light-powered Escherichia coli by co-expression of Gloeobacter rhodopsin expression.Microbial cell factories · 2023Article
- Article
- Application of direct electrometry in studies of microbial rhodopsins reconstituted in proteoliposomes.Biophysical reviews · 2022Review
- Carotenoid binding in Gloeobacteria rhodopsin provides insights into divergent evolution of xanthorhodopsin types.Communications biology · 2022Article
- Coupling a Live Cell Directed Evolution Assay with Coevolutionary Landscapes to Engineer an Improved Fluorescent Rhodopsin Chloride Sensor.ACS synthetic biology · 2022Article
- The Role of Selected Wavelengths of Light in the Activity of Photosystem II inInternational journal of molecular sciences · 2021Article
- A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride.Chemical science · 2021Article
- Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization.Nature communications · 2021Article
- Article
- The chirality origin of retinal-carotenoid complex in gloeobacter rhodopsin: a temperature-dependent excitonic coupling.Scientific reports · 2020Article
- X-ray Crystallographic Structure and Oligomerization of Gloeobacter Rhodopsin.Scientific reports · 2019Article
- Functional importance of the oligomer formation of the cyanobacterial HScientific reports · 2019Article
- Redshifted and Near-infrared Active Analog Pigments Based upon Archaerhodopsin-3.Photochemistry and photobiology · 2019Article
- Functional Expression ofFrontiers in bioengineering and biotechnology · 2019Article
- Functional characterization of sodium-pumping rhodopsins with different pumping properties.PloS one · 2017Article
- Structurally Distinct Cation Channelrhodopsins from Cryptophyte Algae.Biophysical journal · 2016Article
- Conversion of a light-driven proton pump into a light-gated ion channel.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the photocurrents of a cyanobacterial rhodopsin Gloeobacter violaceus (GR) in Xenopus laevis oocytes and HEK-293 cells. This protein is a light-driven proton pump with striking similarities to marine proteorhodopsins, including the D121-H87 cluster of the retinal Schiff base counterion and a glutamate at position 132 that acts as a proton donor for chromophore reprotonation during the photocycle. Interestingly, at low extracellular pH(o) and negative voltage, the proton flux inverted and directed inward. Using electrophysiological measurements of wild-type and mutant GR, we demonstrate that the electrochemical gradient limits outward-directed proton pumping and converts it into a purely passive proton influx. This conclusion contradicts the contemporary paradigm that at low pH, proteorhodopsins actively transport H(+) into cells. We identified E132 and S77 as key residues that allow inward directed diffusion. Substitution of E132 with aspartate or S77 with either alanine or cysteine abolished the inward-directed current almost completely. The proton influx is likely caused by the pK(a) of E132 in GR, which is lower than that of other microbial ion pumping rhodopsins. The advantage of such a low pK(a) is an acceleration of the photocycle and high pump turnover at high light intensities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.