ReviewAntioxidants & redox signaling2014
Fluorescence-based force/tension sensors: a novel tool to visualize mechanical forces in structural proteins in live cells.
Review in Antioxidants & redox signaling, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer's Disease Diagnosis.Micromachines · 2025Article
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Myosin II tension sensors visualize force generation within the actin cytoskeleton in living cells.Journal of cell science · 2024Article
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Recent developments in DNA-based mechanical nanodevices.Chemical communications (Cambridge, England) · 2022Review
- Ultrasound Mediated Cellular Deflection Results in Cellular Depolarization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Force From Filaments: The Role of the Cytoskeleton and Extracellular Matrix in the Gating of Mechanosensitive Channels.Frontiers in cell and developmental biology · 2022Review
- Article
- Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology.Critical reviews in biomedical engineering · 2020Review
- A Toolbox for Organelle Mechanobiology Research-Current Needs and Challenges.Micromachines · 2019Article
- Prediction of Sub-Monomer A2 Domain Dynamics of the von Willebrand Factor by Machine Learning Algorithm and Coarse-Grained Molecular Dynamics Simulation.Scientific reports · 2019Article
- Biomechanics of Collective Cell Migration in Cancer Progression: Experimental and Computational Methods.ACS biomaterials science & engineering · 2019Article
- Application of the fluctuation theorem to motor proteins: from FBiophysical reviews · 2018Review
- A Membrane-Bound Biosensor Visualizes Shear Stress-Induced Inhomogeneous Alteration of Cell Membrane Tension.iScience · 2018Article
- Post-Turing tissue pattern formation: Advent of mechanochemistry.PLoS computational biology · 2018Article
- Voltage-gated and stretch-activated potassium channels in the human heart : Pathophysiological and clinical significance.Herzschrittmachertherapie & Elektrophysiologie · 2018Review
- Inferring cellular forces from image stacks.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Mechanical stress activates NMDA receptors in the absence of agonists.Scientific reports · 2017Article
- Regulation of Intracellular Structural Tension by Talin in the Axon Growth and Regeneration.Molecular neurobiology · 2016Article
- Cardiac Mechano-Gated Ion Channels and Arrhythmias.Circulation research · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
significanceThree signaling systems, chemical, electrical, and mechanical, ubiquitously contribute to cellular activities. There is limited information on the mechanical signaling system because of a lack of tools to measure stress in specific proteins. Although significant advances in methodologies such as atomic force microscopy and laser tweezers have achieved great success in single molecules and measuring the mean properties of cells and tissues, they cannot deal with specific proteins in live cells. RECENT ADVANCES: To remedy the situation, we developed a family of genetically encoded optical force sensors to measure the stress in structural proteins in living cells. The sensors can be incorporated into specific proteins and are not harmful in transgenic animals. The chimeric proteins distribute and function as their wild-type counterparts, and local stress can be read out from changes in Förster resonance energy transfer (FRET). CRITICAL ISSUES: Our original sensor used two mutant green fluorescence proteins linked by an alpha helix that served as a linking spring. Ever since, we have improved the probe design in a number of ways. For example, we replaced the helical linker with more common elastic protein domains to better match the compliance of the wild-type hosts. We greatly improved sensitivity by using the angular dependence of FRET rather than the distance dependence as the transduction mechanism, because that has nearly 100% efficiency at rest and nearly zero when stretched. FUTURE DIRECTIONS: These probes enable researchers to investigate the roles of mechanical force in cellular activities at the level of single molecules, cells, tissues, and whole animals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.