ArticlePloS one2013
Contact hypersensitivity to oxazolone provokes vulvar mechanical hyperalgesia in mice.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Exploring Localized Provoked Vulvodynia: Insights from Animal Model Research.International journal of molecular sciences · 2024Pooled it
- The Involvement of Glutamate-mGluR5 Signaling in the Development of Vulvar Hypersensitivity.International journal of molecular sciences · 2025Article
- Immune mechanisms in vulvodynia: key roles for mast cells and fibroblasts.Frontiers in cellular and infection microbiology · 2023Review
- Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats.Journal of inflammation research · 2022Article
- mMCP7, a Mouse Ortholog of δ Tryptase, Mediates Pelvic Tactile Allodynia in a Model of Chronic Pelvic Pain.Frontiers in pain research (Lausanne, Switzerland) · 2021Article
- Article
- Repeated Vaginal Exposures to the Common Cosmetic and Household Preservative Methylisothiazolinone Induce Persistent, Mast Cell-Dependent Genital Pain in ND4 Mice.International journal of molecular sciences · 2019Article
- Tetrahydrocannabinol Reduces Hapten-Driven Mast Cell Accumulation and Persistent Tactile Sensitivity in Mouse Model of Allergen-Provoked Localized Vulvodynia.International journal of molecular sciences · 2019Article
- The Influence of Early Life Experience on Visceral Pain.Frontiers in systems neuroscience · 2018Review
- Recurrent Yeast Infections and Vulvodynia: Can We Believe Associations Based on Self-Reported Data?Journal of women's health (2002) · 2017Article
- Repeated hapten exposure induces persistent tactile sensitivity in mice modeling localized provoked vulvodynia.PloS one · 2017Article
- Effect of Epac1 on pERK and VEGF Activation in Postoperative Persistent Pain in Rats.Journal of molecular neuroscience : MN · 2016Article
- Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation.Journal of visualized experiments : JoVE · 2016Article
- New topical treatment of vulvodynia based on the pathogenetic role of cross talk between nociceptors, immunocompetent cells, and epithelial cells.Journal of pain research · 2016Article
- Mast Cell-Mediated Mechanisms of Nociception.International journal of molecular sciences · 2015Review
- Mast cells: versatile gatekeepers of pain.Molecular immunology · 2015Review
- Clonal differences in IgE antibodies affect cutaneous anaphylaxis-associated thermal sensitivity in mice.Immunology letters · 2014Article
- Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The interplay among pain, allergy and dysregulated inflammation promises to yield significant conceptual advances in immunology and chronic pain. Hapten-mediated contact hypersensitivity reactions are used to model skin allergies in rodents but have not been utilized to study associated changes in pain perception in the affected skin. Here we characterized changes in mechanical hyperalgesia in oxazolone-sensitized female mice challenged with single and repeated labiar skin exposure to oxazolone. Female mice were sensitized with topical oxazolone on their flanks and challenged 1-3 times on the labia. We then measured mechanical sensitivity of the vulvar region with an electronic pressure meter and evaluated expression of inflammatory genes, leukocyte influx and levels of innervation in the labiar tissue. Oxazolone-sensitized mice developed vulvar mechanical hyperalgesia after a single labiar oxazolone challenge. Hyperalgesia lasted up to 24 hours along with local influx of neutrophils, upregulation of inflammatory cytokine gene expression, and increased density of cutaneous labiar nerve fibers. Three daily oxazolone challenges produced vulvar mechanical hyperalgesic responses and increases in nerve density that were detectable up to 5 days post-challenge even after overt inflammation resolved. This persistent vulvar hyperalgesia is resonant with vulvodynia, an understudied chronic pain condition that is remarkably prevalent in 18-60 year-old women. An elevated risk for vulvodynia has been associated with a history of environmental allergies. Our pre-clinical model can be readily adapted to regimens of chronic exposures and long-term assessment of vulvar pain with and without concurrent inflammation to improve our understanding of mechanisms underlying subsets of vulvodynia and to develop new therapeutics for this condition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.