ArticlePloS one2013
Colon cryptogenesis: asymmetric budding.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Sequential Patterning of Photoresponsive Hydrogels Directs Crypt Fission and Reveals the Role of Epithelial Curvature on Fission Symmetry.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- PHYFUM: Phylogenetic Reconstruction of Normal and Pre-malignant Tissue Evolution Using Fluctuating Methylation.bioRxiv : the preprint server for biology · 2026Article
- Integrating artificial intelligence (AI) into colorectal cancer reporting.The Journal of pathology · 2026Review
- Impact of sample handling protocols on soft tissue attenuation coefficient and morphology.Journal of biomedical optics · 2025Article
- WNT4 promotes the symmetric fission of crypt in radiation-induced intestinal epithelial regeneration.Cellular & molecular biology letters · 2024Article
- Article
- The spatial transcriptomic landscape of the healing mouse intestine following damage.Nature communications · 2022Article
- 3D cyclorama for digital unrolling and visualisation of deformed tubes.Scientific reports · 2021Article
- Azoxymethane-Induced Colorectal Cancer Mice Treated with a Polyphenol-Rich Apple Extract Show Less Neoplastic Lesions and Signs of Cachexia.Foods (Basel, Switzerland) · 2021Article
- Development and organization of the zebrafish intestinal epithelial stem cell niche.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Impaired Wnt/β-catenin pathway leads to dysfunction of intestinal regeneration during necrotizing enterocolitis.Cell death & disease · 2019Article
- E-cadherin Beyond Structure: A Signaling Hub in Colon Homeostasis and Disease.International journal of molecular sciences · 2019Review
- Review
- Mechanistic Insights into Colorectal Cancer Phenomics from Fundamental and Organotypic Model Studies.The American journal of pathology · 2018Review
- Mapping the physical network of cellular interactions.Nature methods · 2018Article
- Colon organoid formation and cryptogenesis are stimulated by growth factors secreted from myofibroblasts.PloS one · 2018Article
- The G-protein coupled receptor 56, expressed in colonic stem and cancer cells, binds progastrin to promote proliferation and carcinogenesis.Oncotarget · 2017Article
- Corrupted colonic crypt fission in carcinogen-treated rats.PloS one · 2017Article
- Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer.BMC cancer · 2016Article
- Paneth Cell-Rich Regions Separated by a Cluster of Lgr5+ Cells Initiate Crypt Fission in the Intestinal Stem Cell Niche.PLoS biology · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The process of crypt formation and the roles of Wnt and cell-cell adhesion signaling in cryptogenesis are not well described; but are important to the understanding of both normal and cancer colon crypt biology. A quantitative 3D-microscopy and image analysis technique is used to study the frequency, morphology and molecular topography associated with crypt formation. Measurements along the colon reveal the details of crypt formation and some key underlying biochemical signals regulating normal colon biology. Our measurements revealed an asymmetrical crypt budding process, contrary to the previously reported symmetrical fission of crypts. 3D immunofluorescence analyses reveals heterogeneity in the subcellular distribution of E-cadherin and β-catenin in distinct crypt populations. This heterogeneity was also found in asymmetrical budding crypts. Singular crypt formation (i.e. no multiple new crypts forming from one parent crypt) were observed in crypts isolated from the normal colon mucosa, suggestive of a singular constraint mechanism to prevent aberrant crypt production. The technique presented improves our understanding of cryptogenesis and suggests that excess colon crypt formation occurs when Wnt signaling is perturbed (e.g. by truncation of adenomatous polyposis coli, APC protein) in most colon cancers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.