ArticlePloS one2013
Novel tools to analyze the function of Salmonella effectors show that SvpB ectopic expression induces cell cycle arrest in tumor cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
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- Microscope Subcellular Localization of Plant-Interacting Bacterial Effectors in Animal Cell Cultures.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Multitask Approach to Localize Rhizobial Type Three Secretion System Effector Proteins Inside Eukaryotic Cells.Plants (Basel, Switzerland) · 2023Article
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- The ADP-Ribosylating Toxins ofToxins · 2019Review
- Host Cell Death Responses to Non-typhoidalFrontiers in immunology · 2019Review
- The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.Microbial biotechnology · 2017Review
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- Effector proteins support the asymmetric apportioning of Salmonella during cytokinesis.Virulence · 2016Article
- Engineering Salmonella as intracellular factory for effective killing of tumour cells.Scientific reports · 2016Article
- Clustered Intracellular Salmonella enterica Serovar Typhimurium Blocks Host Cell Cytokinesis.Infection and immunity · 2016Article
- Improved cytotoxic effects of Salmonella-producing cytosine deaminase in tumour cells.Microbial biotechnology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to further characterize its role in pathogenesis and to establish whether its overproduction can lead to eukaryotic tumor cell death, Salmonella strains able to express its virulence factor SpvB (an ADP-ribosyl transferase enzyme) in a salicylate-inducible way have been constructed and analyzed in different eukaryotic tumor cell lines. To do so, the bacterial strains bearing the expression system have been constructed in a ∆purD background, which allows control of bacterial proliferation inside the eukaryotic cell. In the absence of bacterial proliferation, salicylate-induced SpvB production resulted in activation of caspases 3 and 7 and apoptotic cell death. The results clearly indicated that controlled SpvB production leads to F-actin depolimerization and either G1/S or G2/M phase arrest in all cell lines tested, thus shedding light on the function of SpvB in Salmonella pathogenesis. In the first place, the combined control of protein production by salicylate regulated vectors and bacterial growth by adenine concentration offers the possibility to study the role of Salmonella effectors during eukaryotic cells infection. In the second place, the salicylate-controlled expression of SpvB by the bacterium provides a way to evaluate the potential of other homologous or heterologous proteins as antitumor agents, and, eventually to construct novel potential tools for cancer therapy, given that Salmonella preferentially proliferates in tumors.
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