ArticlePloS one2013
Microfluidic purification and concentration of malignant pleural effusions for improved molecular and cytomorphological diagnostics.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Additive diagnostic value of liquid‑based preparation and conventional smears in Bethesda I thyroid nodules.American journal of clinical pathology · 2026Article
- Fluid-Derived Organoids from Pleural Effusion and Ascites: Emerging Models for Drug Resistance and Personalized Oncology.Journal of Cancer · 2026Review
- Effects of Shear and Extensional Stresses on Cells: Investigation in a Spiral Microchannel and Contraction-Expansion Arrays.ACS biomaterials science & engineering · 2025Review
- Research on the Method of Detecting TPN-Labeled Tumor Cells in Pleural Effusion Based on the Microfluidic Chip.Micromachines · 2024Article
- Vortex sorting of rare particles/cells in microcavities: A review.Biomicrofluidics · 2024Review
- High-Efficiency Inertial Separation of Microparticles Using Elevated Columned Reservoirs and Vortex Technique for Lab-on-a-Chip Applications.ACS omega · 2023Article
- A Microfluidic System for Detecting Tumor Cells Based on Biomarker Hexaminolevulinate (HAL): Applications in Pleural Effusion.Micromachines · 2023Article
- [A Method for Isolating Tumor Cells from Large Volume of Malignant Pleural Effusion and Its Efficacy Evaluation].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2020Article
- Stiffness based enrichment of leukemia cells using microfluidics.APL bioengineering · 2020Article
- A Review of Secondary Flow in Inertial Microfluidics.Micromachines · 2020Review
- Label-free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion.Oncology letters · 2019Article
- Separation of cancer cells using vortical microfluidic flows.Biomicrofluidics · 2018Article
- Materials and microfluidics: enabling the efficient isolation and analysis of circulating tumour cells.Chemical Society reviews · 2017Review
- Label-free isolation of prostate circulating tumor cells using Vortex microfluidic technology.NPJ precision oncology · 2017Article
- Article
- Classification of large circulating tumor cells isolated with ultra-high throughput microfluidic Vortex technology.Oncotarget · 2016Article
- Microfluidic devices to enrich and isolate circulating tumor cells.Lab on a chip · 2015Review
- Inertial particle separation by differential equilibrium positions in a symmetrical serpentine micro-channel.Scientific reports · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evaluation of pleural fluids for metastatic cells is a key component of diagnostic cytopathology. However, a large background of smaller leukocytes and/or erythrocytes can make accurate diagnosis difficult and reduce specificity in identification of mutations of interest for targeted anti-cancer therapies. Here, we describe an automated microfluidic system (Centrifuge Chip) which employs microscale vortices for the size-based isolation and concentration of cancer cells and mesothelial cells from a background of blood cells. We are able to process non-diluted pleural fluids at 6 mL/min and enrich target cells significantly over the background; we achieved improved purity in all patient samples analyzed. The resulting isolated and viable cells are readily available for immunostaining, cytological analysis, and detection of gene mutations. To demonstrate the utility towards aiding companion diagnostics, we also show improved detection accuracy of KRAS gene mutations in lung cancer cells processed using the Centrifuge Chip, leading to an increase in the area under the curve (AUC) of the receiver operating characteristic from 0.90 to 0.99. The Centrifuge Chip allows for rapid concentration and processing of large volumes of bodily fluid samples for improved cytological diagnosis and purification of cells of interest for genetic testing, which will be helpful for enhancing diagnostic accuracy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.