ArticlePloS one2013
Biochemical characterization of a first fungal esterase from Rhizomucor miehei showing high efficiency of ester synthesis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- Expression and characterization of a Clostridium Butyricum esterase from pit cellar of Chinese liquor and its potential in flavor ester synthesis.Archives of microbiology · 2025Article
- Mycotransformation of Commercial Grade Cypermethrin Dispersion byMolecules (Basel, Switzerland) · 2024Article
- Unravelling the hidden power of esterases for biomanufacturing of short-chain esters.Scientific reports · 2023Article
- The Toxicity of Salicylhydroxamic Acid and Its Effect on the Sensitivity ofJournal of fungi (Basel, Switzerland) · 2022Article
- Characterization and purification of esterase from Cellulomonas fimi DB19 isolated from Zanthoxylum armatum with its possible role in diesel biodegradation.Archives of microbiology · 2022Article
- Structure-Guided Engineering of a Family IV Cold-Adapted Esterase Expands Its Substrate Range.International journal of molecular sciences · 2022Article
- Characterization of a new bifunctional endo-1,4-β-xylanase/esterase found in the rumen metagenome.Scientific reports · 2021Article
- Biochemical characterization of an esterase from Clostridium acetobutylicum with novel GYSMG pentapeptide motif at the catalytic domain.Journal of industrial microbiology & biotechnology · 2020Article
- Biodiesel and flavor compound production using a novel promiscuous cold-adapted SGNH-type lipase (Biotechnology for biofuels · 2020Article
- The first description of a hormone-sensitive lipase from a basidiomycete: Structural insights and biochemical characterization revealed Bjerkandera adusta BaEstB as a novel esterase.MicrobiologyOpen · 2017Article
- Engineered Production of Short Chain Fatty Acid in Escherichia coli Using Fatty Acid Synthesis Pathway.PloS one · 2016Article
- Structural insights into the substrate specificity of two esterases from the thermophilic Rhizomucor miehei.Journal of lipid research · 2015Article
- Genome sequence and transcriptome analyses of the thermophilic zygomycete fungus Rhizomucor miehei.BMC genomics · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEsterases with excellent merits suitable for commercial use in ester production field are still insufficient. The aim of this research is to advance our understanding by seeking for more unusual esterases and revealing their characterizations for ester synthesis. METHODOLOGY/PRINCIPAL
findingsA novel esterase-encoding gene from Rhizomucor miehei (RmEstA) was cloned and expressed in Escherichia coli. Sequence analysis revealed a 975-bp ORF encoding a 324-amino-acid polypeptide belonging to the hormone-sensitive lipase (HSL) family IV and showing highest similarity (44%) to the Paenibacillus mucilaginosus esterase/lipase. Recombinant RmEstA was purified to homogeneity: it was 34 kDa by SDS-PAGE and showed optimal pH and temperature of 6.5 and 45°C, respectively. The enzyme was stable to 50°C, under a broad pH range (5.0-10.6). RmEstA exhibited broad substrate specificity toward p-nitrophenol esters and short-acyl-chain triglycerols, with highest activities (1,480 U mg(-1) and 228 U mg(-1)) for p-nitrophenyl hexanoate and tributyrin, respectively. RmEstA efficiently synthesized butyl butyrate (92% conversion yield) when immobilized on AOT-based organogel.
conclusionRmEstA has great potential for industrial applications. RmEstA is the first reported esterase from Rhizomucor miehei.
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