ArticlePloS one2013
Tol2-mediated generation of a transgenic haplochromine cichlid, Astatotilapia burtoni.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Complex pectoral-fin-driven locomotion underlies refined visuomotor behavior in the cichlidiScience · 2026Article
- The neuroendocrine basis for parental care in teleost fish.Current opinion in neurobiology · 2026Review
- Cichlid fish as a model for understanding social dysfunction.Current opinion in neurobiology · 2026Review
- Highly efficient transgenesis mediated by Tip100 transposon system in medaka.Transgenic research · 2025Article
- A pheromone receptor in cichlid fish mediates attraction to females but inhibits male parental care.Current biology : CB · 2024Article
- The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: II. Establishment of a microinjection procedure for genetic engineering.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: I. Fin ray ontogeny and postembryonic staging.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Genome editing in East African cichlids and tilapias: state-of-the-art and future directions.Open biology · 2023Review
- Morphological and temporal variation in early embryogenesis contributes to species divergence in Malawi cichlid fishes.Evolution & development · 2023Article
- East African cichlid fishes.EvoDevo · 2023Review
- Manipulation of the Tyrosinase gene permits improved CRISPR/Cas editing and neural imaging in cichlid fish.Scientific reports · 2021Article
- Dark world rises: The emergence of cavefish as a model for the study of evolution, development, behavior, and disease.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2020Article
- Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Comprehensive Experimental System for a Promising Model Organism Candidate for Marine Teleosts.Scientific reports · 2019Article
- The skeletal ontogeny of Astatotilapia burtoni - a direct-developing model system for the evolution and development of the teleost body plan.BMC developmental biology · 2018Article
- The Value of Comparative Animal Research: Krogh's Principle Facilitates Scientific Discoveries.Policy insights from the behavioral and brain sciences · 2018Article
- Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus) - towards understanding gene function and regulatory evolution in an ecological model system for rapid phenotypic diversification.BMC developmental biology · 2017Article
- Transposons As Tools for Functional Genomics in Vertebrate Models.Trends in genetics : TIG · 2017Review
- Polygenic sex determination in the cichlid fish Astatotilapia burtoni.BMC genomics · 2016Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cichlid fishes represent one of the most species-rich and rapid radiations of a vertebrate family. These ~2200 species, predominantly found in the East African Great Lakes, exhibit dramatic differences in anatomy, physiology, and behavior. However, the genetic bases for this radiation, and for the control of their divergent traits, are unknown. A flood of genomic and transcriptomic data promises to suggest mechanisms underlying the diversity, but transgenic technology will be needed to rigorously test the hypotheses generated. Here we demonstrate the successful use of the Tol2 transposon system to generate transgenic Astatotilapia burtoni, a haplochromine cichlid from Lake Tanganyika, carrying the GFP transgene under the control of the ubiquitous EF1α promoter. The transgene integrates into the genome, is successfully passed through the germline, and the widespread GFP expression pattern is stable across siblings and multiple generations. The stable inheritance and expression patterns indicate that the Tol2 system can be applied to generate A. burtoni transgenic lines. Transgenesis has proven to be a powerful technology for manipulating genes and cells in other model organisms and we anticipate that transgenic A. burtoni and other cichlids will be used to test the mechanisms underlying behavior and speciation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.