ArticlePloS one2013
Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Hotspot pocket-based discovery of urea transporter selective inhibitors.Nature communications · 2026Article
- Identification and Validation of Urea Transporter B Inhibitor fromMolecules (Basel, Switzerland) · 2025Article
- Energy-Dependent Urea Transports in Mammals and their Functional Consequences.Sub-cellular biochemistry · 2025Review
- Physiological Functions of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Urea Transporters and Their Gene Mutations in Diseases.Sub-cellular biochemistry · 2025Review
- Protein Structures of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Computational exploration ofBiochemistry and biophysics reports · 2024Article
- Amine-reactive crosslinking enhances type 0 collagen hydrogel properties for regenerative medicine.Frontiers in bioengineering and biotechnology · 2024Article
- SLC14A1 is a new biomarker in renal cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2023Article
- Study on the role of SLC14A1 gene in biochemical recurrence of prostate cancer.Scientific reports · 2022Article
- A guide to plasma membrane solute carrier proteins.The FEBS journal · 2021Review
- SLC14A1 (UT-B) gene rearrangement in urothelial carcinoma of the bladder: a case report.Diagnostic pathology · 2020Article
- The urea transporter UT-A1 plays a predominant role in a urea-dependent urine-concentrating mechanism.The Journal of biological chemistry · 2020Article
- SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.Theranostics · 2020Article
- Osmotic regulation of UT-B urea transporters in the RT4 human urothelial cell line.Physiological reports · 2019Article
- Physiological functions of urea transporter B.Pflugers Archiv : European journal of physiology · 2019Review
- Urea transport B gene induces melanoma B16 cell death via activation of p53 and mitochondrial apoptosis.Cancer science · 2018Article
- Effect of Cross-Linking Density on the Structures and Properties of Carbodiimide-Treated Gelatin Matrices as Limbal Stem Cell Niches.International journal of molecular sciences · 2018Article
- SLC14A1: a novel target for human urothelial cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2017Review
- WDR23 regulates NRF2 independently of KEAP1.PLoS genetics · 2017Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrevious studies found that urea transporter UT-B is abundantly expressed in bladder urothelium. However, the dynamic role of UT-B in bladder urothelial cells remains unclear. The objective of this study is to evaluate the physiological roles of UT-B in bladder urothelium using UT-B knockout mouse model and T24 cell line. METHODOLOGY/PRINCIPAL
findingsUrea and NO measurement, mRNA expression micro-array analysis, light and transmission electron microscopy, apoptosis assays, DNA damage and repair determination, and intracellular signaling examination were performed in UT-B null bladders vs wild-type bladders and in vitro T24 epithelial cells. UT-B was highly expressed in mouse bladder urothelium. The genes, Dcaf11, MCM2-4, Uch-L1, Bnip3 and 45 S pre rRNA, related to DNA damage and apoptosis were significantly regulated in UT-B null urothelium. DNA damage and apoptosis highly occurred in UT-B null urothelium. Urea and NO levels were significantly higher in UT-B null urothelium than that in wild-type, which may affect L-arginine metabolism and the intracellular signals related to DNA damage and apoptosis. These findings were consistent with the in vitro study in T24 cells that, after urea loading, exhibited cell cycle delay and apoptosis. CONCLUSIONS/SIGNIFICANCE: UT-B may play an important role in protecting bladder urothelium by balancing intracellular urea concentration. Disruption of UT-B function induces DNA damage and apoptosis in bladder, which can result in bladder disorders.
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