ArticlePLoS genetics2013
High throughput sequencing reveals alterations in the recombination signatures with diminishing Spo11 activity.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Rapid identification of reproductive toxicants among environmental chemicals using an in vivo evaluation of gametogenesis in budding yeast Saccharomyces cerevisiae.Reproductive toxicology (Elmsford, N.Y.) · 2024Article
- Meiosis in budding yeast.Genetics · 2023Article
- The courtship choreography of homologous chromosomes: timing and mechanisms of DSB-independent pairing.Frontiers in cell and developmental biology · 2023Review
- The Msh5 complex shows homeostatic localization in response to DNA double-strand breaks in yeast meiosis.Frontiers in cell and developmental biology · 2023Article
- Time to match; when do homologous chromosomes become closer?Chromosoma · 2022Article
- Article
- A role for synaptonemal complex in meiotic mismatch repair.Genetics · 2022Article
- Sex without crossing over in the yeast Saccharomycodes ludwigii.Genome biology · 2021Article
- The synaptonemal complex central region modulates crossover pathways and feedback control of meiotic double-strand break formation.Nucleic acids research · 2021Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2021Article
- HO Endonuclease-Initiated Recombination in Yeast Meiosis Fails To Promote Homologous Centromere Pairing and Is Not Constrained To Utilize the Dmc1 Recombinase.G3 (Bethesda, Md.) · 2018Article
- The Number of Meiotic Double-Strand Breaks Influences Crossover Distribution in Arabidopsis.The Plant cell · 2018Article
- Review
- Exiting prophase I: no clear boundary.Current genetics · 2018Review
- Tetrad analysis in plants and fungi finds large differences in gene conversion rates but no GC bias.Nature ecology & evolution · 2018Article
- Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis.The EMBO journal · 2017Article
- Synaptonemal Complex Proteins of Budding Yeast Define Reciprocal Roles in MutSγ-Mediated Crossover Formation.Genetics · 2016Article
- Sordaria, a model system to uncover links between meiotic pairing and recombination.Seminars in cell & developmental biology · 2016Review
- Modeling meiotic chromosome pairing: nuclear envelope attachment, telomere-led active random motion, and anomalous diffusion.Physical biology · 2016Article
- Extensive Recombination of a Yeast Diploid Hybrid through Meiotic Reversion.PLoS genetics · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Spo11 is the topoisomerase-like enzyme responsible for the induction of the meiosis-specific double strand breaks (DSBs), which initiates the recombination events responsible for proper chromosome segregation. Nineteen PCR-induced alleles of SPO11 were identified and characterized genetically and cytologically. Recombination, spore viability and synaptonemal complex (SC) formation were decreased to varying extents in these mutants. Arrest by ndt80 restored these events in two severe hypomorphic mutants, suggesting that ndt80-arrested nuclei are capable of extended DSB activity. While crossing-over, spore viability and synaptonemal complex (SC) formation defects correlated, the extent of such defects was not predictive of the level of heteroallelic gene conversions (prototrophs) exhibited by each mutant. High throughput sequencing of tetrads from spo11 hypomorphs revealed that gene conversion tracts associated with COs are significantly longer and gene conversion tracts unassociated with COs are significantly shorter than in wild type. By modeling the extent of these tract changes, we could account for the discrepancy in genetic measurements of prototrophy and crossover association. These findings provide an explanation for the unexpectedly low prototroph levels exhibited by spo11 hypomorphs and have important implications for genetic studies that assume an unbiased recovery of prototrophs, such as measurements of CO homeostasis. Our genetic and physical data support previous observations of DSB-limited meioses, in which COs are disproportionally maintained over NCOs (CO homeostasis).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.