ArticlePLoS genetics2013
Systematic unraveling of the unsolved pathway of nicotine degradation in Pseudomonas.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 127 citations in OpenAlex.
- Proximity-enhanced co-immobilized enzyme cascade for efficient bioconversion of nicotine to 3-succinoylpyridine.Bioresources and bioprocessing · 2026Article
- Bacteria in the Phylum Bacillota Degrade Nicotine Through the Variant of Pyridine and Pyrrolidine Pathway.Current microbiology · 2025Article
- Reduction of nicotine content in tobacco through microbial degradation: research progress and potential applications.Biotechnology for biofuels and bioproducts · 2024Review
- Substrate specificity of a branch of aromatic dioxygenases determined by three distinct motifs.Nature communications · 2024Article
- Metagenome-assembled bacterial genomes derived from various aging flue-cured tobacco samples.Microbiology resource announcements · 2024Article
- Rational Design of a Flavoenzyme for Aerobic Nicotine Catabolism.bioRxiv : the preprint server for biology · 2024Article
- Genomic and Metagenomic Insights into the Distribution of Nicotine-degrading Enzymes in Human Microbiota.Current genomics · 2024Article
- A multifunctional flavoprotein monooxygenase HspB for hydroxylation and C-C cleavage of 6-hydroxy-3-succinoyl-pyridine.Applied and environmental microbiology · 2024Article
- Directed evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme.Nature chemical biology · 2023Article
- A conserved hymenopteran-specific family of cytochrome P450s protects bee pollinators from toxic nectar alkaloids.Science advances · 2023Article
- Binding Interface and Electron Transfer Between Nicotine Oxidoreductase and Its Cytochrome c Electron Acceptor.Biochemistry · 2022Article
- The Novel Monooxygenase GeneApplied and environmental microbiology · 2022Article
- The Leaf Microbiome of Tobacco Plants across Eight Chinese Provinces.Microorganisms · 2022Article
- Investigation of indole biodegradation by Cupriavidus sp. strain IDO with emphases on downstream biotransformation and indigo production.Environmental science and pollution research international · 2022Article
- Nicotine metabolism pathway in bacteria: mechanism, modification, and application.Applied microbiology and biotechnology · 2022Review
- Biodegradation of aromatic pollutants meets synthetic biology.Synthetic and systems biotechnology · 2021Article
- Hexabromocyclododecanes Are Dehalogenated by CYP168A1 fromApplied and environmental microbiology · 2021Article
- A Type VI Secretion System Facilitates Fitness, Homeostasis, and Competitive Advantages for Environmental Adaptability and Efficient Nicotine Biodegradation.Applied and environmental microbiology · 2021Article
- A cold shock protein promotes high-temperature microbial growth through binding to diverse RNA species.Cell discovery · 2021Article
- A cytochrome c is the natural electron acceptor for nicotine oxidoreductase.Nature chemical biology · 2021Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microorganisms such as Pseudomonas putida play important roles in the mineralization of organic wastes and toxic compounds. To comprehensively and accurately elucidate key processes of nicotine degradation in Pseudomonas putida, we measured differential protein abundance levels with MS-based spectral counting in P. putida S16 grown on nicotine or glycerol, a non-repressive carbon source. In silico analyses highlighted significant clustering of proteins involved in a functional pathway in nicotine degradation. The transcriptional regulation of differentially expressed genes was analyzed by using quantitative reverse transcription-PCR. We observed the following key results: (i) The proteomes, containing 1,292 observed proteins, provide a detailed view of enzymes involved in nicotine metabolism. These proteins could be assigned to the functional groups of transport, detoxification, and amino acid metabolism. There were significant differences in the cytosolic protein patterns of cells growing in a nicotine medium and those in a glycerol medium. (ii) The key step in the conversion of 3-succinoylpyridine to 6-hydroxy-3-succinoylpyridine was catalyzed by a multi-enzyme reaction consisting of a molybdopeterin binding oxidase (spmA), molybdopterin dehydrogenase (spmB), and a (2Fe-2S)-binding ferredoxin (spmC) with molybdenum molybdopterin cytosine dinucleotide as a cofactor. (iii) The gene of a novel nicotine oxidoreductase (nicA2) was cloned, and the recombinant protein was characterized. The proteins and functional pathway identified in the current study represent attractive targets for degradation of environmental toxic compounds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.