ArticlePLoS genetics2013
Mobility of the native Bacillus subtilis conjugative plasmid pLS20 is regulated by intercellular signaling.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 79 citations in OpenAlex.
- Invasive plasmids as ecosystem engineers-from mechanism to application.Essays in biochemistry · 2026Review
- Expression of conjugation genes is controlled by a novel zipper-type transcriptional attenuation mechanism.Nucleic acids research · 2026Article
- A roadmap to understanding and anticipating microbial gene transfer in soil communities.Microbiology and molecular biology reviews : MMBR · 2025Review
- Role of horizontal gene transfer and cooperation in rhizosphere microbiome assembly.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Review
- Integrated conjugal plasmid pLS20 in the Bacillus subtilis genome produced 850-kbp circular subgenomes transmissible to another B. subtilis.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- Modelling Plasmid-Mediated Horizontal Gene Transfer in Biofilms.Bulletin of mathematical biology · 2024Article
- Effect of TraN key residues involved in DNA binding on pIP501 transfer rates inFrontiers in molecular biosciences · 2024Article
- Major distinctions between the two oligopeptide permease systems ofMicrobiology (Reading, England) · 2023Article
- Extraordinary long-stem confers resistance of intrinsic terminators to processive antitermination.Nucleic acids research · 2023Article
- Cell-to-cell non-conjugative plasmid transfer between Bacillus subtilis and lactic acid bacteria.Microbial biotechnology · 2023Article
- A tetramerization domain in prokaryotic and eukaryotic transcription regulators homologous to p53.Acta crystallographica. Section D, Structural biology · 2023Article
- The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues.Nucleic acids research · 2022Article
- A New Tool for the Flexible Genetic Manipulation ofBio-protocol · 2022Article
- A New Variant of theAntibiotics (Basel, Switzerland) · 2022Article
- A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation.Microbial cell factories · 2022Article
- Conjugation Operons in Gram-Positive Bacteria with and without Antitermination Systems.Microorganisms · 2022Article
- pLS20 is the archetype of a new family of conjugative plasmids harboured byNAR genomics and bioinformatics · 2021Article
- Assessment ofMicroorganisms · 2021Article
- A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria.Nucleic acids research · 2021Article
- A native conjugative plasmid confers potential selective advantages to plant growth-promoting Bacillus velezensis strain GH1-13.Communications biology · 2021Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Horizontal gene transfer mediated by plasmid conjugation plays a significant role in the evolution of bacterial species, as well as in the dissemination of antibiotic resistance and pathogenicity determinants. Characterization of their regulation is important for gaining insights into these features. Relatively little is known about how conjugation of Gram-positive plasmids is regulated. We have characterized conjugation of the native Bacillus subtilis plasmid pLS20. Contrary to the enterococcal plasmids, conjugation of pLS20 is not activated by recipient-produced pheromones but by pLS20-encoded proteins that regulate expression of the conjugation genes. We show that conjugation is kept in the default "OFF" state and identified the master repressor responsible for this. Activation of the conjugation genes requires relief of repression, which is mediated by an anti-repressor that belongs to the Rap family of proteins. Using both RNA sequencing methodology and genetic approaches, we have determined the regulatory effects of the repressor and anti-repressor on expression of the pLS20 genes. We also show that the activity of the anti-repressor is in turn regulated by an intercellular signaling peptide. Ultimately, this peptide dictates the timing of conjugation. The implications of this regulatory mechanism and comparison with other mobile systems are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.