ArticlePlant physiology2014
Coexpressing Escherichia coli cyclopropane synthase with Sterculia foetida Lysophosphatidic acid acyltransferase enhances cyclopropane fatty acid accumulation.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- A toolkit for plant lipid engineering: Surveying the efficacies of lipogenic factors for accumulating specialty lipids.Frontiers in plant science · 2022Pooled it
- Insights into Chemical Diversity and Potential Health-Promoting Effects of Ferns.Plants (Basel, Switzerland) · 2024Review
- Exploring engineering strategies that enhance de novo production of exotic cyclopropane fatty acids in Saccharomyces cerevisiae.Biotechnology journal · 2024Article
- Engineering triacylglycerol accumulation in duckweed (Lemna japonica).Plant biotechnology journal · 2023Article
- Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in YeastJournal of fungi (Basel, Switzerland) · 2022Review
- Co-expressingMetabolic engineering communications · 2021Article
- Biotin attachment domain-containing proteins mediate hydroxy fatty acid-dependent inhibition of acetyl CoA carboxylase.Plant physiology · 2021Article
- Transcriptome Analysis and Identification of Lipid Genes inInternational journal of molecular sciences · 2021Article
- Fermentation process for producing CFAs using Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2020Article
- Producing Cyclopropane Fatty Acid in Plant Leafy BiomassFrontiers in plant science · 2020Article
- Expression of a LycheePlant physiology · 2019Article
- Biosynthesis, regulation, and engineering of microbially produced branched biofuels.Biotechnology for biofuels · 2019Review
- Omics-based analyses revealed metabolic responses ofBiotechnology for biofuels · 2019Article
- Cyclopropane fatty acid biosynthesis in plants: phylogenetic and biochemical analysis of Litchi Kennedy pathway and acyl editing cycle genes.Plant cell reports · 2018Article
- Seed-specific RNAi in safflower generates a superhigh oleic oil with extended oxidative stability.Plant biotechnology journal · 2018Article
- Structural and Functional Analysis of E. coli Cyclopropane Fatty Acid Synthase.Structure (London, England : 1993) · 2018Article
- Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil.Plant biotechnology journal · 2018Article
- Article
- Increased Serum Level of Cyclopropaneoctanoic Acid 2-Hexyl in Patients with Hypertriglyceridemia-Related Disorders.Lipids · 2016Article
- Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclopropane fatty acids (CPAs) are desirable as renewable chemical feedstocks for the production of paints, plastics, and lubricants. Toward our goal of creating a CPA-accumulating crop, we expressed nine higher plant cyclopropane synthase (CPS) enzymes in the seeds of fad2fae1 Arabidopsis (Arabidopsis thaliana) and observed accumulation of less than 1% CPA. Surprisingly, expression of the Escherichia coli CPS gene resulted in the accumulation of up to 9.1% CPA in the seed. Coexpression of a Sterculia foetida lysophosphatidic acid acyltransferase (SfLPAT) increases CPA accumulation up to 35% in individual T1 seeds. However, seeds with more than 9% CPA exhibit wrinkled seed morphology and reduced size and oil accumulation. Seeds with more than 11% CPA exhibit strongly decreased seed germination and establishment, and no seeds with CPA more than 15% germinated. That previous reports suggest that plant CPS prefers the stereospecific numbering (sn)-1 position whereas E. coli CPS acts on sn-2 of phospholipids prompted us to investigate the preferred positions of CPS on phosphatidylcholine (PC) and triacylglycerol. Unexpectedly, in planta, E. coli CPS acts primarily on the sn-1 position of PC; coexpression of SfLPAT results in the incorporation of CPA at the sn-2 position of lysophosphatidic acid. This enables a cycle that enriches CPA at both sn-1 and sn-2 positions of PC and results in increased accumulation of CPA. These data provide proof of principle that CPA can accumulate to high levels in transgenic seeds and sets the stage for the identification of factors that will facilitate the movement of CPA from PC into triacylglycerol to produce viable seeds with additional CPA accumulation.
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