ArticleNucleic acids research2014
High-throughput, cost-effective verification of structural DNA assembly.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Computer-Aided Planning for the Verification of Large Batches of DNA Constructs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Engineering biological systems using automated biofoundries.Metabolic engineering · 2017Review
- Registry in a tube: multiplexed pools of retrievable parts for genetic design space exploration.Nucleic acids research · 2017Article
- Rapid prototyping of microbial cell factories via genome-scale engineering.Biotechnology advances · 2015Review
- Pydna: a simulation and documentation tool for DNA assembly strategies using python.BMC bioinformatics · 2015Article
- Recent advances in DNA assembly technologies.FEMS yeast research · 2015Review
- Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria.Molecular systems biology · 2014Article
- Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA 'assembly' from 'building blocks' remains a cornerstone in synthetic biology, whether it be for gene synthesis (∼ 1 kb), pathway engineering (∼ 10 kb) or synthetic genomes (>100 kb). Despite numerous advances in the techniques used for DNA assembly, verification of the assembly is still a necessity, which becomes cost-prohibitive and a logistical challenge with increasing scale. Here we describe for the first time a comprehensive, high-throughput solution for structural DNA assembly verification by restriction digest using exhaustive in silico enzyme screening, rolling circle amplification of plasmid DNA, capillary electrophoresis and automated digest pattern recognition. This low-cost and robust methodology has been successfully used to screen over 31 000 clones of DNA constructs at <$1 per sample.
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Registered trials
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