ArticleNucleic acids research2014
The ZorO-OrzO type I toxin-antitoxin locus: repression by the OrzO antitoxin.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Type I toxin-antitoxin systems in bacteria: from regulation to biological functions.EcoSal Plus · 2024Review
- A simple BLASTn-based approach generates novel insights into the regulation and biological function of type I toxin-antitoxins.mSystems · 2024Article
- An RNA pseudoknot mediates toxin translation and antitoxin inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Toxin-antitoxin systems in bacterial pathogenesis.Heliyon · 2023Review
- Charged Amino Acids Contribute to ZorO Toxicity.Toxins · 2022Article
- T1TAdb: the database of type I toxin-antitoxin systems.RNA (New York, N.Y.) · 2021Article
- Improved growth ofJournal of bacteriology · 2021Article
- RNA Regulated Toxin-Antitoxin Systems in Pathogenic Bacteria.Frontiers in cellular and infection microbiology · 2021Review
- An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli.Nucleic acids research · 2020Article
- Lrg1 Regulates β (1,3)-Glucan Masking in Candida albicans through the Cek1 MAP Kinase Pathway.mBio · 2019Article
- Article
- Toxin⁻Antitoxin Systems inToxins · 2019Review
- Type I Toxin-Antitoxin Systems in Clostridia.Toxins · 2019Review
- Type I Toxin-Antitoxin Systems: Regulating Toxin Expression via Shine-Dalgarno Sequence Sequestration and Small RNA Binding.Microbiology spectrum · 2018Review
- Discovery of new type I toxin-antitoxin systems adjacent to CRISPR arrays in Clostridium difficile.Nucleic acids research · 2018Article
- RNA-based regulation in type I toxin-antitoxin systems and its implication for bacterial persistence.Current genetics · 2017Review
- Mechanistic insights into type I toxin antitoxin systems in Helicobacter pylori: the importance of mRNA folding in controlling toxin expression.Nucleic acids research · 2017Article
- The 5΄ UTR of the type I toxin ZorO can both inhibit and enhance translation.Nucleic acids research · 2017Article
- Article
- Microcystin-LR does not induce alterations to transcriptomic or metabolomic profiles of a model heterotrophic bacterium.PloS one · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type I toxin-antitoxin loci consist of two genes: a small, hydrophobic, potentially toxic protein, and a small RNA (sRNA) antitoxin. The sRNA represses toxin gene expression by base pairing to the toxin mRNA. A previous bioinformatics search predicted a duplicated type I locus within Escherichia coli O157:H7 (EHEC), which we have named the gene pairs zorO-orzO and zorP-orzP. We show that overproduction of the zorO gene is toxic to E. coli; co-expression of the sRNA OrzO can neutralize this toxicity, confirming that the zorO-orzO pair is a true type I toxin-antitoxin locus. However, OrzO is unable to repress zorO in a strain deleted for RNase III, indicating that repression requires cleavage of the target mRNA. Sequence analysis and mutagenesis studies have elucidated a nucleotide sequence region (V1) that allows differential recognition of the zorO mRNA by OrzO and not OrzP, and a specific single nucleotide within the V1 of OrzO that is critical for repression of zorO. Although there are 18 nt of complementarity between the OrzO sRNA and the zorO mRNA, not all base pairing interactions are needed for repression; however, the amount needed is dependent on whether there is continuous or discontinuous complementarity to the target mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.