ArticleMethods in molecular biology (Clifton, N.J.)2014
Multiprotein complex production in insect cells by using polyproteins.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- pFastBac4x: A Novel Vector for the Simultaneous Expression of Four Proteins-Expression and Purification of a G-Protein Complex as a Case Study.Molecular biotechnology · 2026Article
- Structural characterization of the oligomerization of full-length Hantaan virus polymerase into symmetric dimers and hexamers.Nature communications · 2024Article
- Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication.Nature communications · 2023Article
- Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription.Nature communications · 2022Article
- Efficient production of protein complexes in mammalian cells using a poxvirus vector.PloS one · 2022Article
- A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells.Protein expression and purification · 2021Review
- Cryo-EM structure of metazoan TRAPPIII, the multi-subunit complex that activates the GTPase Rab1.The EMBO journal · 2021Article
- MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells.Viruses · 2019Review
- The two TRAPP complexes of metazoans have distinct roles and act on different Rab GTPases.The Journal of cell biology · 2018Article
- Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain.Molecular biotechnology · 2018Article
- Review
- MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes.Methods in enzymology · 2017Article
- Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen.Biotechnology and bioengineering · 2016Article
- biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Highly efficient baculovirus-mediated multigene delivery in primary cells.Nature communications · 2016Article
- Structural characterization of recombinant IAV polymerase reveals a stable complex between viral PA-PB1 heterodimer and host RanBP5.Scientific reports · 2016Article
- The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.Advances in experimental medicine and biology · 2016Review
- Polyproteins in structural biology.Current opinion in structural biology · 2015Review
- Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.Nature communications · 2015Article
- Baculovirus expression: old dog, new tricks.Bioengineered · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A powerful approach utilizing polyproteins for balancing stoichiometry of recombinant multiprotein complexes overproduced in baculovirus expression vector systems (BEVS) is described. This procedure has been implemented here in the MultiBac system but can also be directly adapted to all commonly used BEVS. The protocol details the design principles of polyprotein-expressing DNA constructs, the generation of composite baculovirus for polyprotein production, and the expression and in vivo processing of polyproteins in baculovirus infected insect cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.