Evidence map›Paper›PMID 24202194›Full record

ArticleAnalytical and bioanalytical chemistry2014

Differentiation of Escherichia coli serotypes using DC gradient insulator dielectrophoresis.

Paul V Jones, Alexa F DeMichele, LaKeta Kemp, Mark A Hayes

Abstract read
In one paragraph

Article in Analytical and bioanalytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Article
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  7. Analytics in Microfluidic Systems.Advances in biochemical engineering/biotechnology · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Paul V JonesDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604, AZ, USA.
Alexa F DeMichele
LaKeta Kemp
Mark A Hayes

Funding

PLATFORM FOR ISOLATION AND CONCENTRATION OF BIOMARKERSR21EB010191 · NIBIB · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI HAYES, MARK A · 2010 to 2011
$407k
Pathogen Isolation and Concentration for Phenotypic SubtypingR03AI099740 · NIAID · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI HAYES, MARK A · 2012 to 2013
$138k
NIAID NIH HHS R03 AI099740NIBIB NIH HHS R21 EB010191
6 · The paper itself

Abstract

Bacteria play a significant role in both human health and disease. An estimated 9.4 million cases of foodborne illness occur in the United States each year. As a result, rapid identification and characterization of microorganisms remains an important research objective. Despite limitations, selective culturing retains a central role among a cadre of identification strategies. For the past decade, separations-based approaches to rapid bacterial identification have been under investigation. Gradient insulator dielectrophoresis (g-iDEP) promises benefits in the form of rapid and specific separation of very similar bacteria, including serotypes of a single species. Furthermore, this approach allows simultaneous concentration of analyte, facilitating detection and downstream analysis. Differentiation of three serotypes or strains of Escherichia coli bacteria is demonstrated within a single g-iDEP microchannel, based on their characteristic electrokinetic properties. Whole cells were captured and concentrated using a range of applied potentials, which generated average electric fields between 160 and 470 V/cm. Bacteria remained viable after exposure to these fields, as determined by cellular motility. These results indicate the potential g-iDEP holds in terms of both separatory power and the possibility for diagnostic applications.

Indexed as

Electric ConductivityElectricityElectrophoresisEscherichia coliMicrofluidic Analytical TechniquesMovementSerotyping

Identifiers

PMID24202194
PMCPMC3935418

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.